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Reference Detail
| Reference | ||
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| Author | Viana V.E., Carlos da Maia L., Busanello C., Pegoraro C., Costa de Oliveira A. | |
| Title | When rice gets the chills: comparative transcriptome profiling at germination shows WRKY transcription factor responses. | |
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Abstract: Rice is vital for food security. Due to its tropical origin, rice suffers from cold temperatures that affect its entire life cycle. Key genes have been identified involved in cold tolerance. WRKYs are generally downstream of the MAPK cascade and can act together with VQ proteins to regulate stress-responsive genes. Chilling treatment was applied at germination to two rice genotypes (tolerant and sensitive). Shoots at S3 stage were collected for RNA-seq to identify OsWRKY, OsMAPKs and OsVQs expression. Relationships among MAPKs, WRKYs and VQs were predicted through correlation analysis. OsWRKY transcriptional regulation was predicted by in silico analysis of cis-regulatory elements. A total of 39 OsWRKYs were differentially expressed. OsWRKY21, OsWRK24 and OsWRKY69 are potential positive regulators, while OsWRKY10, OsWRK47, OsWRKY62, OsWRKY72 and OsWRKY77 are potential negative regulators, of chilling tolerance. 12 OsMAPKs were differentially expressed. OsMAPKs were downregulated and negatively correlated with the upregulated OsWRKYs in the tolerant genotype. 19 OsVQs were differentially expressed, three and six OsVQs were positively correlated with OsWRKYs in the tolerant and sensitive genotypes, respectively. Seven differentially expressed OsWRKYs have cold-responsive elements in their promoters and five upregulated OsWRKYs in the tolerant genotype contained the W-box motif. Chilling causes changes in OsWRKY, OsMAPK and OsVQ gene expression at germination. OsWRKYs may not act downstream of the MAPK cascade to coordinate chilling tolerance, but OsWRKYs may act with VQs to regulate chilling tolerance. Candidate OsWRKYs are correlated and have a W-box in the promoter, suggesting an auto-regulation mechanism. |
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| Journal | Plant Biol (Stuttg) | |
| Country | Brazil | |
| Volume | 23 Suppl 1 | |
| Pages | 100-112 | |
| Year | 2021 | |
| PubMed ID | 33773005 | |
| PubMed Central ID | - | |
| DOI | 10.1111/plb.13262 | |
| URL | - | |
| Relation | ||
| Gene | DSG1 MAP3 MAPK4 MPK6 MPK7 MPK9 VQ1 VQ11 VQ13 VQ16 VQ19 VQ24 VQ25 VQ30 VQ35 VQ5 VQ9 WRKY10 WRKY21 WRKY24 WRKY3 WRKY42 WRKY47 WRKY53 WRKY62 WRKY69 WRKY71 WRKY72 WRKY74 WRKY76 WRKY77 WRKY8 _ _ | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
