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Reference Detail
| Reference | ||
|---|---|---|
| Author | Huang K., Wu T., Ma Z., Li Z., Chen H., Zhang M., Bian M., Bai H., Jiang W., Du X. | |
| Title | Rice Transcription Factor OsWRKY55 Is Involved in the Drought Response and Regulation of Plant Growth. | |
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Abstract: WRKY transcription factors (TFs) have been reported to respond to biotic and abiotic stresses and regulate plant growth and development. However, the molecular mechanisms of WRKY TFs involved in drought stress and regulating plant height in rice remain largely unknown. In this study, we found that transgenic rice lines overexpressing <i>OsWRKY55</i> (<i>OsWRKY55</i>-OE) exhibited reduced drought resistance. The <i>OsWRKY55</i>-OE lines showed faster water loss and greater accumulation of hydrogen peroxide (H2O2) and superoxide radical (O2-·) compared to wild-type (WT) plants under drought conditions. <i>OsWRKY55</i> was expressed in various tissues and was induced by drought and abscisic acid (ABA) treatments. Through yeast two-hybrid assays, we found that OsWRKY55 interacted with four mitogen-activated protein kinases (MAPKs) that could be induced by drought, including OsMPK7, OsMPK9, OsMPK20-1, and OsMPK20-4. The activation effects of the four OsMPKs on OsWRKY55 transcriptional activity were demonstrated by a GAL4-dependent chimeric transactivation assay in rice protoplasts. Furthermore, OsWRKY55 was able to reduce plant height under normal conditions by decreasing the cell size. In addition, based on a dual luciferase reporter assay, OsWRKY55 was shown to bind to the promoter of <i>OsAP2-39</i> through a yeast one-hybrid assay and positively regulate <i>OsAP2-39</i> expression. These results suggest that OsWRKY55 plays a critical role in responses to drought stress and the regulation of plant height in rice, further providing valuable information for crop improvement. |
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| Journal | Int J Mol Sci | |
| Country | China | |
| Volume | 22(9) | |
| Pages | ||
| Year | 2021 | |
| PubMed ID | 33919349 | |
| PubMed Central ID | 8122443 | |
| DOI | 10.3390/ijms22094337 | |
| URL | - | |
| Relation | ||
| Gene | AP2/EREBP129 DLN24 ERF48 ERF77 FBOX516 MPK20-1 MPK7 MPK9 MYB48 RING327 WJUMK1 WRKY55 ZFP15 _ | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
