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Reference Detail
| Reference | ||
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| Author | Niu B., Zhang Z., Zhang J., Zhou Y., Chen C. | |
| Title | The rice LEC1-like transcription factor OsNF-YB9 interacts with SPK, an endosperm-specific sucrose synthase protein kinase, and functions in seed development. | |
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Abstract: LEAFY COTYLEDON1 (LEC1), a NUCLEAR FACTOR-Y (NF-Y) family member, plays a critical role in embryogenesis and seed development in Arabidopsis. Previous studies have shown that rice OsNF-YB9 and OsNF-yb7 are homologous to Arabidopsis LEC1. However, the functions of LEC1-like genes in rice remain unclear. Here we report that OsNF-YB9 and OsNF-yb7 display sub-functionalization in rice. We demonstrate that OsNF-yb7 is expressed mainly in the embryo, whereas OsNF-YB9 is preferentially expressed in the developing endosperm. Heterologous expression of either OsNF-YB9 or OsNF-yb7 in Arabidopsis LEC1-1 was able to complement the LEC1-1 defects. We failed to generate OsNF-yb7 homozygous mutants due to lethality caused by OsNF-yb7 defects. Loss of OsNF-YB9 function caused abnormal seed development: seeds were longer, narrower and thinner and exhibited a higher chalkiness ratio. Furthermore, the expression of genes related to starch synthesis was deregulated in OsNF-YB9. OsNF-YB9 could interact with SPK, a sucrose synthase protein kinase that is predominantly expressed in rice endosperm. Knockout of SPK resulted in chalky seeds similar to those observed in the OsNF-YB9 mutants. Ectopic expression of OsNF-YB9 in both rice and Arabidopsis resulted in unhealthy plants with small seeds. Taken together, these results suggest a critical role for OsNF-YB9 in rice seed development. |
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| Journal | Plant J. | |
| Country | China | |
| Volume | 106(5) | |
| Pages | 1233-1246 | |
| Year | 2021 | |
| PubMed ID | 33721364 | |
| PubMed Central ID | - | |
| DOI | 10.1111/tpj.15230 | |
| URL | - | |
| Relation | ||
| Gene | AGPL1 AGPS2 FLO5 HAP3D ISA2 LEC1 PUL SBE1 SBE3 SPK SSI SUT1 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
