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Reference Detail
| Reference | ||
|---|---|---|
| Author | Ga E., Song J., Min M.K., Ha J., Park S., Lee S.B., Lee J.Y., Kim B.G. | |
| Title | Reconstitution of Cytokinin Signaling in Rice Protoplasts. | |
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Abstract: The major components of the cytokinin (CK) signaling pathway have been identified from the receptors to their downstream transcription factors. However, since signaling proteins are encoded by multigene families, characterizing and quantifying the contribution of each component or their combinations to the signaling cascade have been challenging. Here, we describe a transient gene expression system in rice (<i>Oryza sativa</i>) protoplasts suitable to reconstitute CK signaling branches using the CK reporter construct <i>TCSn:fLUC</i>, consisting of a synthetic CK-responsive promoter and the firefly luciferase gene, as a sensitive readout of signaling output. We used this system to systematically test the contributions of CK signaling components, either alone or in various combinations, with or without CK treatment. The type-B response regulators (RRs) OsRR16, OsRR17, OsRR18, and OsRR19 all activated <i>TCSn:fLUC</i> strongly, with OsRR18 and OsRR19 showing the strongest induction by CK. Cotransfecting the reporter with <i>OsHP01</i>, <i>OsHP02</i>, <i>OsHP05</i>, or <i>OsHK03</i> alone resulted in much weaker effects relative to those of the type-B OsRRs. When we tested combinations of OsHK03, OsHPs, and OsRRs, each combination exhibited distinct CK signaling activities. This system thus allows the rapid and high-throughput exploration of CK signaling in rice. |
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| Journal | Int J Mol Sci | |
| Country | South Korea | |
| Volume | 22(7) | |
| Pages | ||
| Year | 2021 | |
| PubMed ID | 33807467 | |
| PubMed Central ID | 8037374 | |
| DOI | 10.3390/ijms22073647 | |
| URL | - | |
| Relation | ||
| Gene | AHP1 AHP2 DLC1 HK3 PHP2 RR21 RR23 RR26 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
