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Reference Detail
| Reference | ||
|---|---|---|
| Author | XING Bao, CHEN Zhen-hua, ZHANG Zhi-guo | |
| Title | Map-based Cloning of a Curly Leaf Gene CLF1 in Rice | |
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Abstract: Leaf is one of the most important photosynthetic organs and plays an important part in the productivity. We screened the rice leaf morphological variation mutants and isolated a curly leaf mutant(clf1)showing abaxial leaf rolling. The more bulliform cells numbers on leaf adaxial sides in clf1 mutnat were the main reason leading to leaves curling. Using Map-Based cloning methods，the site controlling CLF1 gene was mapped in chromosome2，tightly linking with two polymorphic markers(InDel51 and InDel57). There were 44 genes in this region and we predicted LOC_OS02G45250 as the target gene by bioinformatics. We found that there were 20bp deletion in the sixth exon of LOC_OS02G45250 and the nucleotide deletion created a premature stop codon in the predicted coding region. The clf1 was another allele of the reported rice curly leaf gene Roc5(Rice outermost cell-specific gene5)，whose encoded transcription factor including GL2 homologous structure domain. Different from the oul1 mutant(loss-of-function in Roc5)，the clf1 was valuable for the production because of a better agricultural characters. |
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| Journal | BIOTECHNOLOGY BULLETIN | |
| Country | China | |
| Volume | 33(9) | |
| Pages | 110-115 | |
| Year | 2017 | |
| PubMed ID | - | |
| PubMed Central ID | - | |
| DOI | 10.13560/j.cnki.biotech.bull.1985.2017-0694 | |
| URL | - | |
| Relation | ||
| Gene | OUL1 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
