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Reference Detail
| Reference | ||
|---|---|---|
| Author | Hwang O.J., Back K. | |
| Title | Suppression of Rice cryptochrome 1b Decreases Both Melatonin and Expression of Brassinosteroid Biosynthetic Genes Resulting in Salt Tolerance. | |
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Abstract: We investigated the relationship between the blue-light photoreceptor cryptochrome (CRY) and melatonin biosynthesis by generating RNA interference (RNAi) transgenic rice plants that suppress the <i>cryptochrome 1b</i> gene (<i>CRY1b</i>). The resulting <i>CRY1b</i> RNAi rice lines expressed less <i>CRY1b</i> mRNA, but not <i>CRY1a</i> or <i>CRY2</i> mRNA, suggesting that the suppression is specific to <i>CRY1b</i>. The growth of <i>CRY1b</i> RNAi rice seedlings was enhanced under blue light compared to wild-type growth, providing phenotypic evidence for impaired CRY function. When these <i>CRY1b</i> RNAi rice plants were challenged with cadmium to induce melatonin, wild-type plants produced 100 ng/g fresh weight (FW) melatonin, whereas <i>CRY1b</i> RNAi lines produced 60 ng/g FW melatonin on average, indicating that melatonin biosynthesis requires the CRY photoreceptor. Due to possible feedback regulation, the expression of melatonin biosynthesis genes such as <i>T5H</i>, <i>SNAT1</i>, <i>SNAT2</i>, and <i>COMT</i> was elevated in the <i>CRY1b</i> RNAi lines compared to the wild-type plants. In addition, laminar angles decreased in the <i>CRY1b</i> RNAi lines via the suppression of brassinosteroid (BR) biosynthesis genes such as <i>DWARF</i>. The main cause of the BR decrease in the <i>CRY1b</i> RNAi lines seems to be the suppression of CRY rather than decreased melatonin because the melatonin decrease suppressed <i>DWARF4</i> rather than <i>DWARF</i>. |
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| Journal | Molecules | |
| Country | South Korea | |
| Volume | 26(4) | |
| Pages | ||
| Year | 2021 | |
| PubMed ID | 33670642 | |
| PubMed Central ID | 7922549 | |
| DOI | 10.3390/molecules26041075 | |
| URL | - | |
| Relation | ||
| Gene | BRD1 BZR1 CPD1 CPD2 CRY1B D61 DET2;2 DWARF4 ROMT9 SL SNAT1 SNAT2 XTH23 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
