Update info.Tools - Reference - Detail
Reference Detail
| Reference | ||
|---|---|---|
| Author | Uzair M., Long H., Zafar S.A., Patil S.B., Chun Y., Li L., Fang J., Zhao J., Peng L., Yuan S., Li X. | |
| Title | Narrow Leaf21, Encoding Ribosomal Protein RPS3A, Controls Leaf Development in Rice. | |
|
Abstract: Leaf morphology influences photosynthesis, transpiration and ultimately crop yield. However, the molecular mechanism of leaf development is still not fully understood. Here, we identified and characterized the narrow leaf21 (nal21) mutant in rice (Oryza sativa), showing a significant reduction in leaf width, leaf length and plant height, and increased tiller number. Microscopic observation revealed defects in the vascular system and reduced epidermal cell size and number in the nal21 leaf blade. Map-based cloning revealed that nal21 encodes a ribosomal small subunit protein RPS3A. Ribosome-targeting antibiotics resistance assay and ribosome profiling showed a significant reduction in the free 40S ribosome subunit in the nal21 mutant. The nal21 mutant showed aberrant auxin responses in which multiple auxin response factors (ARFs) harboring upstream open reading frames (uORFs) in their 5'-untranslated region (5'-UTR) were repressed at the translational level. The WUSCHEL-related homeobox 3A (OsWOX3A) gene, a key transcription factor involved in leaf blade lateral outgrowth, is also under the translational regulation by RPS3A. Transformation with modified OsARF11, OsARF16 and OsWOX3A genomic DNA lacking uORFs rescued the narrow leaf phenotype of nal21 to a better extent than transformation with their native genomic DNA, implying that RPS3A could regulate translation of ARFs and WOX3A through uORFs. Our results demonstrate that proper translational regulation of key factors involved in leaf development is essential to maintain normal leaf morphology. |
||
| Journal | Plant Physiol. | |
| Country | China | |
| Volume | ||
| Pages | ||
| Year | 2021 | |
| PubMed ID | 33591317 | |
| PubMed Central ID | - | |
| DOI | 10.1093/plphys/kiab075 | |
| URL | - | |
| Relation | ||
| Gene | ARF16 IAA20 LC1 MP NAL2 NAL21 NAL3 RPS3AB RPS3AC | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
|
Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
