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Author Hu B., Zhou Y., Zhou Z., Sun B., Zhou F., Yin C., Ma W., Chen H., Lin Y.
Title Repressed OsMESL expression triggers reactive oxygen species-mediated broad-spectrum disease resistance in rice.
Abstract:
A few reports have indicated that a single gene confers resistance to bacterial blight, sheath blight and rice blast. In this study, we identified a novel disease resistance mutant gene, methyl esterase-like (OsMESL) in rice. Mutant rice with T-DNA insertion displayed significant resistance to bacterial blight caused by Xanthomonas oryzae pv. oryzae (Xoo), sheath blight caused by Rhizoctonia solani and rice blast caused by Magnaporthe oryzae. Additionally, CRISPR-Cas9 knockout mutants and RNAi lines displayed resistance to these pathogens. Complementary T-DNA mutants demonstrated a phenotype similar to the wild type (WT), thereby indicating that OsMESL confers resistance to pathogens. Protein interaction experiments revealed that OsMESL affects reactive oxygen species (ROS) accumulation by interacting with thioredoxin OsTrxm in rice. Moreover, qRT-PCR results showed significantly reduced mRNA levels of multiple ROS scavenging-related genes in OsMESL mutants. Nitroblue tetrazolium staining showed that the pathogens cause ROS accumulation, and quantitative detection revealed significantly increased levels of H2 O2 in the leaves of OsMESL mutants and RNAi lines after infection. The abundance of JA, a hormone associated with disease resistance, was significantly more in OsMESL mutants than in WT plants. Overall, these results suggested that OsMESL enhances disease resistance to Xoo, R. solani and M. oryzae by modulating the ROS balance.
Journal Plant Biotechnol. J.
Country China
Volume
Pages
Year 2021
PubMed ID 33567155
PubMed Central ID -
DOI 10.1111/pbi.13566
URL -
Relation
Gene AOC AOX1A AOX1B APX1 APX3 APX8 CATA CATB DR8 JAR1 JIOSPR10 JMT1 LOX1 LOX1 LOX2 LOX8 MADS26 MESL MT2B OG1 OPR1 PIZ PR1A PR1B RSOSPR10 SODA1 SODB SPH TRX29 WRKY24 WRKY45 WRKY76 WRKY77 _ _ _
INSD -
Strain Wild Core Collection -
Induced Mutation Lines(NIG Collection) -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain
-
Stages in Each Organ
- Muant Lines (Gene)
-
Cultivated Varieties(NIG Collection) -
Stages in Each Organ -
/rice/oryzabase