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Reference Detail
| Reference | ||
|---|---|---|
| Author | Li Y., Liao S., Mei P., Pan Y., Zhang Y., Zheng X., Xie Y., Miao Y. | |
| Title | <i>OsWRKY93</i> Dually Functions Between leaf senescence and in Response to Biotic Stress in Rice. | |
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Abstract: Cross talking between natural senescence and cell death in response to pathogen attack is an interesting topic; however, its action mechanism is kept open. In this study, 33 <i>OsWRKY</i> genes were obtained by screening with leaf aging procedure through RNA-seq dataset, and 11 of them were confirmed a significant altered expression level in the flag leaves during aging by using the reverse transcript quantitative PCR (RT-qPCR). Among them, the <i>OsWRKY2</i>, <i>OsWRKY14</i>, <i>OsWRKY26</i>, <i>OsWRKY69</i>, and <i>OsWRKY93</i> members exhibited short-term alteration in transcriptional levels in response to <i>Magnaporthe grisea</i> infection. The CRISPR/Cas9-edited mutants of five genes were developed and confirmed, and a significant sensitivity to <i>M. oryzae</i> infection was observed in <i>CRISPR OsWRKY93-edited</i> lines; on the other hand, a significant resistance to <i>M. oryzae</i> infection was shown in the enhanced expression <i>OsWRKY93</i> plants compared to mock plants; however, enhanced expression of other four genes have no significant affection. Interestingly, ROS accumulation was also increased in <i>OsWRKY93</i> enhanced plants after flg22 treatment, compared with the controls, suggesting that <i>OsWRKY93</i> is involved in PAMP-triggered immune response in rice. It indicated that <i>OsWRKY93</i> was involved in both flag leaf senescence and in response to fungi attack. |
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| Journal | Front Plant Sci | |
| Country | China | |
| Volume | 12 | |
| Pages | 643011 | |
| Year | 2021 | |
| PubMed ID | 33828575 | |
| PubMed Central ID | 8019945 | |
| DOI | 10.3389/fpls.2021.643011 | |
| URL | - | |
| Relation | ||
| Gene | WRKY10 WRKY113 WRKY14 WRKY2 WRKY26 WRKY29 WRKY47 WRKY49 WRKY69 WRKY72 WRKY73 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
