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Reference Detail
| Reference | ||
|---|---|---|
| Author | Zhang Z., He Y., Li L., Zhang X., Xu X., Shi Y., Wu J.L. | |
| Title | Characterization of a novel allele encoding pheophorbide a oxygenase in rice. | |
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Abstract: We identified a <i>rapid cell death 2</i> (<i>rcd2</i>) mutant from an indica cultivar Zhongjian100 mutant bank. The red-brown lesions appeared firstly on young seedling leaves, then gradually merged and the leaves completely withered at the late tillering stage. <i>rcd2</i> displayed apparent cell death at/around the lesions, accumulation of superoxide anion (O2-) and disturbed ROS scavenging system, impaired photosynthetic capacity with significantly reduced chlorophyll content. The lesion formation was controlled by a single recessive nuclear gene and induced by natural light as well as mechanical wounding. A single base mutation (A1726T) at the 6th exon of <i>OsMH_03G0040800</i> resulted in I576F substitution in the encoding protein, pheophorbide a oxygenase (PAO). Functional complementation could rescue the mutant phenotype and <i>PAO-</i>knockout lines exhibited the similar phenotype to <i>rcd2</i>. The activity of PAO decreased significantly while the content of PAO substrate, pheophorbide a, increased apparently in <i>rcd2</i>. The expression of chlorophyll synthesis/degradation-related genes and the contents of metabolic intermediates were largely changed. Furthermore, the level of chlorophyllide a, the product of chlorophyllase, increased significantly, indicating chlorophyllase might play a role in chlorophyll degradation in rice. Our results suggested that the I576F substitution disrupted PAO function, leading to O2- accumulation and chlorophyll degradation breakdown in rice. |
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| Journal | Plant Signal Behav | |
| Country | China | |
| Volume | 16(3) | |
| Pages | 1864606 | |
| Year | 2021 | |
| PubMed ID | 33369525 | |
| PubMed Central ID | 7889113 | |
| DOI | 10.1080/15592324.2020.1864606 | |
| URL | - | |
| Relation | ||
| Gene | CHL EAS1 NYC3 SGR SGRL YGL10 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
