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Reference Detail
| Reference | ||
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| Author | Xiao Y., Zhang J., Yu G., Lu X., Mei W., Deng H., Zhang G., Chen G., Chu C., Tong H., Tang W. | |
| Title | Endoplasmic Reticulum-Localized PURINE PERMEASE1 Regulates plant height and grain weight by Modulating Cytokinin Distribution in Rice. | |
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Abstract: Cytokinins (CKs) are a class of phytohormones playing essential roles in various biological processes. However, the mechanisms underlying CK transport as well as its function in plant growth and development are far from being fully elucidated. Here, we characterize the function of PURINE PERMEASE1 (OsPUP1) in rice (<i>Oryza sativa</i> L.). <i>OsPUP1</i> was predominantly expressed in the root, particularly in vascular cells, and CK treatment can induce its expression. Subcellular localization analysis showed that OsPUP1 was predominantly localized to the endoplasmic reticulum (ER). Overexpression of <i>OsPUP1</i> resulted in growth defect of various aerial tissues, including decreased leaf length, plant height, grain weight, panicle length, and grain number. Hormone profiling revealed that the CK content was decreased in the shoot of <i>OsPUP1</i>-overexpressing seedling, but increased in the root, compared with the wild type. The CK content in the panicle was also decreased. Quantitative reverse transcription-PCR (qRT-PCR) analysis using several CK type-A <i>response regulators</i> (<i>OsRRs</i>) as the marker genes suggested that the CK response in the shoot of <i>OsPUP1</i>-overexpressing seedling is decreased compared to the wild type when CKs are applied to the root. Genetic analysis revealed that BG3/OsPUP4, a putative plasma membrane-localized CK transporter, overcomes the function of OsPUP1. We hypothesize that OsPUP1 might be involved in importing CKs into ER to unload CKs from the vascular tissues by cell-to-cell transport. |
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| Journal | Front Plant Sci | |
| Country | China | |
| Volume | 11 | |
| Pages | 618560 | |
| Year | 2020 | |
| PubMed ID | 33414802 | |
| PubMed Central ID | 7783468 | |
| DOI | 10.3389/fpls.2020.618560 | |
| URL | - | |
| Relation | ||
| Gene | PUP1 PUP4 RR1 RR2 RR4 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
