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Reference Detail
| Reference | ||
|---|---|---|
| Author | Fu R., Wang C., Shen H., Zhang J., Higgins J.D., Liang W. | |
| Title | Rice OsBRCA2 Is Required for DNA Double-Strand Break Repair in Meiotic Cells. | |
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Abstract: The mammalian <i>BREAST CANCER 2</i> (<i>BRCA2</i>) gene is a tumor suppressor that plays a crucial role in DNA repair and homologous recombination (HR). Here, we report the identification and characterization of <i>OsBRCA2</i>, the rice orthologue of human <i>BRCA2</i>. <i>OsBRCA2</i> mutant plants exhibit normal vegetative growth but experience complete male and female sterility as a consequence of severe meiotic defects. Pairing, synapsis and recombination are impaired in <i>OsBRCA2</i> male meiocytes, leading to chromosome entanglements and fragmentation. In the absence of OsBRCA2, localization to the meiotic chromosome axes of the strand-invasion proteins OsRAD51 and OsDMC1 is severely reduced and <i>in vitro</i> OsBRCA2 directly interacts with OsRAD51 and OsDMC1. These results indicate that OsBRCA2 is essential for facilitating the loading of OsRAD51 and OsDMC1 onto resected ends of programmed double-strand breaks (DSB) during meiosis to promote single-end invasions of homologous chromosomes and accurate recombination. In addition, treatment of <i>OsBRCA2-1</i> seedlings with mitomycin C (MMC) led to hypersensitivity. As MMC is a genotoxic agent that creates DNA lesions in the somatic cells that can only be repaired by HR, these results suggest that OsBRCA2 has a conserved role in DSB repair and HR in rice. |
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| Journal | Front Plant Sci | |
| Country | China | |
| Volume | 11 | |
| Pages | 600820 | |
| Year | 2020 | |
| PubMed ID | 33304374 | |
| PubMed Central ID | 7701097 | |
| DOI | 10.3389/fpls.2020.600820 | |
| URL | - | |
| Relation | ||
| Gene | BRCA2 COLD11 DMC1A DMC1B RAD51A2 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
