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Reference Detail
| Reference | ||
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| Author | Gho Y.S., Choi H., Moon S., Song M.Y., Park H.E., Kim D.H., Ha S.H., Jung K.H. | |
| Title | Phosphate-Starvation-Inducible S-like RNase Genes in Rice Are Involved in Phosphate Source Recycling by RNA Decay. | |
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Abstract: The fine-tuning of inorganic phosphate (Pi) for enhanced use efficiency has long been a challenging subject in agriculture, particularly in regard to rice as a major crop plant. Among ribonucleases (RNases), the RNase T2 family is broadly distributed across kingdoms, but little has been known on its substrate specificity compared to RNase A and RNase T1 families. Class I and class II of the RNase T2 family are defined as the S-like RNase (RNS) family and have showed the connection to Pi recycling in Arabidopsis. In this study, we first carried out a phylogenetic analysis of eight rice and five Arabidopsis <i>RNS</i> genes and identified mono-specific class I and dicot-specific class I RNS genes, suggesting the possibility of functional diversity between class I RNS family members in monocot and dicot species through evolution. We then compared the <i>in silico</i> expression patterns of all <i>RNS</i> genes in rice and Arabidopsis under normal and Pi-deficient conditions and further confirmed the expression patterns of rice <i>RNS</i> genes via qRT-PCR analysis. Subsequently, we found that most of the <i>OsRNS</i> genes were differentially regulated under Pi-deficient treatment. Association of Pi recycling by RNase activity in rice was confirmed by measuring total RNA concentration and ribonuclease activity of shoot and root samples under Pi-sufficient or Pi-deficient treatment during 21 days. The total RNA concentrations were decreased by < 60% in shoots and < 80% in roots under Pi starvation, respectively, while ribonuclease activity increased correspondingly. We further elucidate the signaling pathway of Pi starvation through upregulation of the <i>OsRNS</i> genes. The 2-kb promoter region of all <i>OsRNS</i> genes with inducible expression patterns under Pi deficiency contains a high frequency of P1BS cis-acting regulatory element (CRE) known as the OsPHR2 binding site, suggesting that the OsRNS family is likely to be controlled by OsPHR2. Finally, the dynamic transcriptional regulation of <i>OsRNS</i> genes by overexpression of <i>OsPHR2</i>, <i>ospho2</i> mutant, and overexpression of <i>OsPT1</i> lines involved in Pi signaling pathway suggests the molecular basis of <i>OsRNS</i> family in Pi recycling via RNA decay under Pi starvation. |
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| Journal | Front Plant Sci | |
| Country | South Korea | |
| Volume | 11 | |
| Pages | 585561 | |
| Year | 2020 | |
| PubMed ID | 33424882 | |
| PubMed Central ID | 7793952 | |
| DOI | 10.3389/fpls.2020.585561 | |
| URL | - | |
| Relation | ||
| Gene | LTN1 PHR2 PT1 RNS1 RNS2 RNS3 RNS4 RNS5 RNS6 RNS7 RNS8 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
