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Reference Detail
| Reference | ||
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| Author | Liu C., Shen Y., Qin B., Wen H., Cheng J., Mao F., Shi W., Tang D., Du G., Li Y., Wu Y., Cheng Z. | |
| Title | <i>Oryza sativa</i> RNA-Dependent RNA polymerase 6 Contributes to Double-Strand Break Formation in Meiosis. | |
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Abstract: RNA-dependent RNA polymerase 6 (RDR6) is a core component of the small RNA biogenesis pathway, but its function in meiosis is unclear. Here, we report a new allele of <i>OsRDR6</i> (<i>OsRDR6-meiosis</i> [<i>OsRDR6-mei</i>]), which causes meiosis-specific phenotypes in rice (<i>Oryza sativa</i>). In <i>OsRDR6-mei</i>, meiotic double-strand break (DSB) formation is partially blocked. We created a biallelic mutant with more severe phenotypes, <i>OsRDR6-bi</i>, by crossing <i>OsRDR6-mei</i> with a knockout mutant, <i>OsRDR6-edit</i> In <i>OsRDR6-bi</i> meiocytes, 24 univalents were observed, and no histone H2AX phosphorylation foci were detected. Compared with the wild type, the number of 21-nucleotide small RNAs in <i>OsRDR6-mei</i> was dramatically lower, while the number of 24-nucleotide small RNAs was significantly higher. Thousands of differentially methylated regions (DMRs) were discovered in <i>OsRDR6-mei</i>, implying that OsRDR6 plays an important role in DNA methylation. There were 457 genes downregulated in <i>OsRDR6-mei</i>, including three genes, <i>CENTRAL REGION COMPONENT1</i>, <i>P31</i> <i>comet</i> , and <i>O. sativa SOLO DANCERS</i>, related to DSB formation. Interestingly, the downregulated genes were associated with a high level of 24-nucleotide small RNAs but less strongly associated with DMRs. Therefore, we speculate that the alteration in expression of small RNAs in <i>OsRDR6</i> mutants leads to the defects in DSB formation during meiosis, which might not be directly dependent on RNA-directed DNA methylation. |
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| Journal | Plant Cell | |
| Country | China | |
| Volume | 32(10) | |
| Pages | 3273-3289 | |
| Year | 2020 | |
| PubMed ID | 32732308 | |
| PubMed Central ID | 7534469 | |
| DOI | 10.1105/tpc.20.00213 | |
| URL | - | |
| Relation | ||
| Gene | CRC1 P31COMET SDS SHL2 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
