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| Reference | ||
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| Author | Wu J., Chen Y., Lin H., Chen Y., Yu H., Lu Z., Li X., Zhou H., Chen Z., Liu X. | |
| Title | Comparative Cytological and Transcriptome Analysis Revealed the Normal pollen Development Process and Up-Regulation of Fertility-Related Genes in Newly Developed Tetraploid Rice. | |
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Abstract: Autotetraploid rice is a useful germplasm for polyploid rice breeding; however, low seed setting is a major hindrance for its utilization. Here, we reported the development of a new tetraploid rice, Huoduo1 (H1), which has the characteristic of high fertility, from crossing generations of autotetraploid rice. Cytological observations displayed the high fertility of the pollen (95.62%) in H1, a lower percentage of pollen mother cell (PMC) abnormalities, and stable chromosome configurations during the pollen development process compared with its parents. Using RNA-seq analysis, we detected 440 differentially expressed genes (DEGs) in H1 compared with its parents. Of these DEGs, 193 were annotated as pollen fertility-related genes, and 129 (~66.8%) exhibited significant up-regulation in H1 compared with the parents, including three environmentally sensitive genic male sterility genes (<i>TMS9-1</i>, <i>TMS5</i>, and <i>CSA</i>), one meiosis gene (<i>RAD51D</i>), and three tapetal-related genes (<i>MIL2</i>, <i>OsAP25</i>, and <i>OsAP37)</i>, which were validated by qRT-PCR in this study. Two genes, <i>TMS9-1</i> and <i>TMS5</i>, were knocked out using CRISPR/Cas9 technology, and their mutants displayed low fertility and the abnormal development of pollen. Our findings provide evidence for the regulatory mechanisms of fertility in tetraploid rice and indicated that the up-regulation of pollen fertility-related genes may contribute to the high fertility in new tetraploid rice. |
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| Journal | Int J Mol Sci | |
| Country | China | |
| Volume | 21(19) | |
| Pages | ||
| Year | 2020 | |
| PubMed ID | 32987934 | |
| PubMed Central ID | 7582553 | |
| DOI | 10.3390/ijms21197046 | |
| URL | - | |
| Relation | ||
| Gene | AP37 C6 CSA DFR2 MIL2 PTC1 RAD51D RADC1 TMS5 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
