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Author Suriyasak C., Oyama Y., Ishida T., Mashiguchi K., Yamaguchi S., Hamaoka N., Iwaya-Inoue M., Ishibashi Y.
Title Mechanism of delayed seed germination caused by high temperature during grain filling in rice (Oryza sativa L.).
High temperature during grain filling considerably reduces yield and quality in rice (Oryza sativa L.); however, how high temperature affects seed germination of the next generation is not yet well understood. Here, we report that seeds from plants exposed to high temperature during the grain filling stage germinated significantly later than seeds from unstressed plants. This delay remained even after dormancy release treatments, suggesting that it was not due to primary seed dormancy determined during grain filling. In imbibed embryos of heat-stressed seeds, expression of abscisic acid (ABA) biosynthesis genes (OsNCEDs) was higher than in those of control seeds, whereas that of ABA catabolism genes (OsABA8'OHs) was lower. In the aleurone layer, despite no change in GA signaling as evidenced by no effect of heat stress on OsGAMYB gene expression, the transcripts of alpha-amylase genes OsAmy1C, OsAmy3B, and OsAmy3E were significantly down-regulated in heat-stressed seeds in comparison with controls. Changes in promoter methylation levels were consistent with transcriptional changes of ABA catabolism-related and alpha-amylase genes. These data suggest that high temperature during grain filling results in DNA methylation of ABA catabolism-related and alpha-amylase gene promoters, delaying germination of heat-stressed seeds.
Journal Sci Rep
Country Japan
Volume 10(1)
Pages 17378
Year 2020
PubMed ID 33060675
PubMed Central ID 7562956
DOI 10.1038/s41598-020-74281-9
Strain Wild Core Collection -
Induced Mutation Lines(NIG Collection) -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain
Stages in Each Organ
- Muant Lines (Gene)
Cultivated Varieties(NIG Collection) -
Stages in Each Organ -