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Reference Detail
| Reference | ||
|---|---|---|
| Author | Zhang Y., Zhao L., Xiao H., Chew J., Xiang J., Qian K., Fan X. | |
| Title | Knockdown of a Novel Gene <i>OsTBP2.2</i> Increases Sensitivity to Drought Stress in Rice. | |
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Abstract: Drought stress is a major environmental stress, which adversely affects the biological and molecular processes of plants, thereby impairing their growth and development. In the present study, we found that the expression level of <i>OsTBP2.2</i> which encodes for a nucleus-localized protein member belonging to transcription factor IID (TFIID) family, was significantly induced by polyethylene glycol (PEG) treatment. Therefore, knockdown mutants of <i>OsTBP2.2</i> gene were generated to investigate the role of OsTBP2.2 in rice response to drought stress. Under the condition of drought stress, the photosynthetic rate, transpiration rate, water use efficiency, and stomatal conductance were significantly reduced in <i>OsTBP2.2</i> lines compared with wild type, Dongjin (WT-DJ). Furthermore, the RNA-seq results showed that several main pathways involved in "MAPK (mitogen-activated protein kinase) signaling pathway", "phenylpropanoid biosynthesis", "defense response" and "ADP (adenosine diphosphate) binding" were altered significantly in <i>OsTBP2.2</i>. We also found that <i>OsPIP2;6</i>, <i>OsPAO</i> and <i>OsRCCR1</i> genes were down-regulated in <i>OsTBP2.2</i> compared with WT-DJ, which may be one of the reasons that inhibit photosynthesis. Our findings suggest that <i>OsTBP2.2</i> may play a key role in rice growth and the regulation of photosynthesis under drought stress and it may possess high potential usefulness in molecular breeding of drought-tolerant rice. |
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| Journal | Genes (Basel) | |
| Country | China | |
| Volume | 11(6) | |
| Pages | ||
| Year | 2020 | |
| PubMed ID | 32521717 | |
| PubMed Central ID | 7349065 | |
| DOI | 10.3390/genes11060629 | |
| URL | - | |
| Relation | ||
| Gene | DREB2A EAS1 GHR1 PIP2;6 PIZT RCCR1 RLCK135 TBP2.2 ZFP6 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
