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Reference Detail
| Reference | ||
|---|---|---|
| Author | Wang S., Chen A., Xie K., Yang X., Luo Z., Chen J., Zeng D., Ren Y., Yang C., Wang L., Feng H., López-Arredondo DL., Herrera-Estrella LR., Xu G. | |
| Title | Functional analysis of the OsNPF4.5 nitrate transporter reveals a conserved mycorrhizal pathway of nitrogen acquisition in plants. | |
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Abstract: Low availability of nitrogen (N) is often a major limiting factor to crop yield in most nutrient-poor soils. Arbuscular mycorrhizal (AM) fungi are beneficial symbionts of most land plants that enhance plant nutrient uptake, particularly of phosphate. A growing number of reports point to the substantially increased N accumulation in many mycorrhizal plants; however, the contribution of AM symbiosis to plant N nutrition and the mechanisms underlying the AM-mediated N acquisition are still in the early stages of being understood. Here, we report that inoculation with AM fungus <i>Rhizophagus irregularis</i> remarkably promoted rice (<i>Oryza sativa</i>) growth and N acquisition, and about 42% of the overall N acquired by rice roots could be delivered via the symbiotic route under N-NO3- supply condition. Mycorrhizal colonization strongly induced expression of the putative nitrate transporter gene <i>OsNPF4.5</i> in rice roots, and its orthologs <i>ZmNPF4.5</i> in <i>Zea mays</i> and <i>SbNPF4.5</i> in <i>Sorghum bicolor</i> OsNPF4.5 is exclusively expressed in the cells containing arbuscules and displayed a low-affinity NO3- transport activity when expressed in <i>Xenopus laevis</i> oocytes. Moreover, knockout of <i>OsNPF4.5</i> resulted in a 45% decrease in symbiotic N uptake and a significant reduction in arbuscule incidence when NO3- was supplied as an N source. Based on our results, we propose that the NPF4.5 plays a key role in mycorrhizal NO3- acquisition, a symbiotic N uptake route that might be highly conserved in gramineous species. |
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| Journal | Proc. Natl. Acad. Sci. U.S.A. | |
| Country | China | |
| Volume | 117(28) | |
| Pages | 16649-16659 | |
| Year | 2020 | |
| PubMed ID | 32586957 | |
| PubMed Central ID | 7368293 | |
| DOI | 10.1073/pnas.2000926117 | |
| URL | - | |
| Relation | ||
| Gene | AMT3;1 NAR2.1 NPF4.5 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
