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| 著者 | Fang C., Zhang P., Li L., Yang L., Mu D., Yan X., Li Z., Lin W. | |
| タイトル | serine hydroxymethyltransferase localised in the endoplasmic reticulum plays a role in scavenging H2O2 to enhance rice chilling tolerance. | |
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Abstract: Rice is a chilling-sensitive crop that would suffer serious damage from low temperatures. Overexpression of the Lsi1 gene (Lsi1-OX) in rice enhances its chilling tolerance. This study revealed that a serine hydroxymethyltransferase (OsSHMT) mainly localised in the endoplasmic reticulum (ER) is involved in increasing tolerance to chilling. A higher transcription level of OsSHMT was detected in Lsi1-OX rice than in the wild type. Histone H1 and nucleic acid binding protein were found to bind to the promoter region of OsSHMT and regulate its expression, and the transcription levels of these proteins were also up-regulated in the Lsi1-OX rice. Moreover, OsSHMT interacts with ATP synthase subunit alpha, heat shock protein Hsp70, mitochondrial substrate carrier family protein, ascorbate peroxidase 1 and ATP synthase subunit beta. Lsi1-encoded protein OsNIP2;1 also interacts with ATP synthase subunit beta, and the coordination of these proteins appears to function in reducing reactive oxygen species, as the H2O2 content of transgenic OsSHMT Arabidopsis thaliana was lower than that of the non-transgenic line under chilling treatment. Our results indicate that ER-localised OsSHMT plays a role in scavenging H2O2 to enhance the chilling tolerance of Lsi1-OX rice and that ATP synthase subunit beta is an intermediate junction between OsNIP2;1 and OsSHMT. |
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| 掲載誌 | BMC Plant Biol. | |
| Country | China | |
| 刊数 | 20(1) | |
| ページ | 236 | |
| 発刊年 | 2020 | |
| PubMed ID | 32456700 | |
| PubMed Central ID | 7249644 | |
| DOI | 10.1186/s12870-020-02446-9 | |
| URL | - | |
| 関連情報 | ||
| 遺伝子 | ALBA3 APX1 ISC24 LSI1 MSCP SHMT1 TUB6 TUBA1 TUBA3 _ _ _ | |
| INSD | - | |
| 系統 |
野生イネ コアコレクション |
- |
| 突然変異系統(遺伝研 Collection) | - | |
| 種子不稔系統 | - | |
| 致死変異体系統 | - | |
|
組織別発生段階 - 突然変異系統(遺伝子) |
- | |
| 栽培イネ品種(遺伝研 Collection) | - | |
| 組織別発生段階 | - | |
