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Reference Detail
| Reference | ||
|---|---|---|
| Author | Park H.L., Yoo Y., Bhoo S.H., Lee T.H., Lee S.W., Cho M.H. | |
| Title | Two Chalcone Synthase Isozymes Participate Redundantly in UV-Induced Sakuranetin Synthesis in Rice. | |
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Abstract: <b>:</b> Chalcone synthase (CHS) is a key enzyme in the flavonoid pathway, participating in the production of phenolic phytoalexins. The rice genome contains 31 <i>CHS</i> family genes (<i>OsCHS</i>s). The molecular characterization of <i>OsCHS</i>s suggests that <i>OsCHS8</i> and <i>OsCHS24</i> belong in the bona fide CHSs, while the other members are categorized in the non-CHS group of type III polyketide synthases (PKSs). Biochemical analyses of recombinant OsCHSs also showed that OsCHS24 and OsCHS8 catalyze the formation of naringenin chalcone from <i>p</i>-coumaroyl-CoA and malonyl-CoA, while the other OsCHSs had no detectable CHS activity. OsCHS24 is kinetically more efficient than OsCHS8. Of the <i>OsCHS</i>s, <i>OsCHS24</i> also showed the highest expression levels in different tissues and developmental stages, suggesting that it is the major CHS isoform in rice. In <i>OsCHS24</i> mutant leaves, sakuranetin content decreased to 64.6% and 80.2% of those in wild-type leaves at 2 and 4 days after UV irradiation, respectively, even though <i>OsCHS24</i> expression was mostly suppressed. Instead, the <i>OsCHS8</i> expression was markedly increased in the <i>OsCHS24</i> mutant under UV stress conditions compared to that in the wild-type, which likely supports the UV-induced production of sakuranetin in <i>OsCHS24</i>. These results suggest that OsCHS24 acts as the main CHS isozyme and OsCHS8 redundantly contributes to the UV-induced production of sakuranetin in rice leaves. |
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| Journal | Int J Mol Sci | |
| Country | South Korea | |
| Volume | 21(11) | |
| Pages | ||
| Year | 2020 | |
| PubMed ID | 32471084 | |
| PubMed Central ID | 7312121 | |
| DOI | 10.3390/ijms21113777 | |
| URL | - | |
| Relation | ||
| Gene | CHS CHS19 CHS26 CHS27 CHS31 LAP6 PKS1 PKS10 PKS11 PKS12 PKS13 PKS14 PKS15 PKS16 PKS17 PKS18 PKS2 PKS20 PKS22 PKS23 PKS24 PKS25 PKS27 PKS3 PKS4 PKS5 PKS6 PKS7 PKS8 PKS9 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
