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Author Ma Z., Wu T., Huang K., Jin Y.M., Li Z., Chen M., Yun S., Zhang H., Yang X., Chen H., Bai H., Du L., Ju S., Guo L., Bian M., Hu L., Du X., Jiang W.
Title A Novel AP2/ERF Transcription Factor, OsRPH1, Negatively Regulates plant height in Rice.
The APETALA 2/ethylene response factors (AP2/ERF) are widespread in the plant kingdom and play essential roles in regulating plant growth and development as well as defense responses. In this study, a novel rice AP2/ERF transcription factor gene, <i>OsRPH1</i>, was isolated and functionally characterized. OsRPH1 falls into group-IVa of the AP2/ERF family. OsRPH1 protein was found to be localized in the nucleus and possessed transcriptional activity. Overexpression of <i>OsRPH1</i> resulted in a decrease in plant height and length of internode and leaf sheath as well as other abnormal characters in rice. The length of the second leaf sheath of <i>OsRPH1</i>-overexpressing (OE) plants recovered to that of Kitaake (non-transgenic recipient) in response to exogenous gibberellin A3 (GA3) application. The expression of GA biosynthesis genes (<i>OsGA20ox1</i>-<i>OsGA20ox4, OsGA3ox1</i>, and <i>OsGA3ox2</i>) was significantly downregulated, whereas that of GA inactivation genes (<i>OsGA2ox7</i>, <i>OsGA2ox9</i>, and <i>OsGA2ox10</i>) was significantly upregulated in <i>OsRPH1-</i>OE plants. Endogenous bioactive GA contents significantly decreased in <i>OsRPH1-</i>OE plants. OsRPH1 interacted with a blue light receptor, OsCRY1b, in a blue light-dependent manner. Taken together, our results demonstrate that OsRPH1 negatively regulates plant height and bioactive GA content by controlling the expression of GA metabolism genes in rice. OsRPH1 is involved in blue light inhibition of leaf sheath elongation by interacting with OsCRY1b.
Journal Front Plant Sci
Country China
Volume 11
Pages 709
Year 2020
PubMed ID 32528516
PubMed Central ID 7266880
DOI 10.3389/fpls.2020.00709
Gene D18 GA20OX3 GA2OX7 GA2OX9 GA3OX1 GNP1 PRL5 RPH1 SD1 _
Strain Wild Core Collection -
Induced Mutation Lines(NIG Collection) -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain
Stages in Each Organ
- Muant Lines (Gene)
Cultivated Varieties(NIG Collection) -
Stages in Each Organ -