Update info.Tools - Reference - Detail
Reference Detail
| Reference | ||
|---|---|---|
| Author | Wierbowski S.D., Vo T.V., Falter-Braun P., Jobe T.O., Kruse L.H., Wei X., Liang J., Meyer M.J., Akturk N., Rivera-Erick CA., Cordero N.A., Paramo M.I., Shayhidin E.E., Bertolotti M., Tippens N.D., Akther K., Sharma R., Katayose Y., Salehi-Ashtiani K., Hao T., Ronald P.C., Ecker J.R., Schweitzer P.A., Kikuchi S., Mizuno H., Hill D.E., Vidal M., Moghe G.D., McCouch S.R., Yu H. | |
| Title | A massively parallel barcoded sequencing pipeline enables generation of the first ORFeome and interactome map for rice. | |
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Abstract: Systematic mappings of protein interactome networks have provided invaluable functional information for numerous model organisms. Here we develop <u>P</u>CR-mediated <u>L</u>inkage of barcoded <u>A</u>dapters <u>T</u>o nucleic acid <u>E</u>lements for <u>seq</u>uencing (PLATE-seq) that serves as a general tool to rapidly sequence thousands of DNA elements. We validate its utility by generating the ORFeome for <i>Oryza sativa</i> covering 2,300 genes and constructing a high-quality protein-protein interactome map consisting of 322 interactions between 289 proteins, expanding the known interactions in rice by roughly 50%. Our work paves the way for high-throughput profiling of protein-protein interactions in a wide range of organisms. |
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| Journal | Proc. Natl. Acad. Sci. U.S.A. | |
| Country | unknown | |
| Volume | 117(21) | |
| Pages | 11836-11842 | |
| Year | 2020 | |
| PubMed ID | 32398372 | |
| PubMed Central ID | 7260977 | |
| DOI | 10.1073/pnas.1918068117 | |
| URL | - | |
| Relation | ||
| Gene | EEF1B EEF1B' FLZ14 GAPC GAPC1 GRX12 OSK4 S5A UBC5 _ | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
