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| Reference | ||
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| Author | Zhang L., Wang C., Yu Y., Zhang Y., Song Y., Li Z., Wang S., Zhang Y., Guo X., Liu D., Li Z., Ma S., Zheng J., Zhao H., Zhang G. | |
| Title | Cyclin-Dependent Kinase Inhibitor gene <i>TaICK1</i> acts as a Potential Contributor to Wheat male sterility induced by a Chemical Hybridizing Agent. | |
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Abstract: Heterosis has been widely accepted as an effective strategy to increase yields in plant breeding. Notably, the chemical hybridization agent SQ-1 induces male sterility in wheat, representing a critical potential tool in hybrid seed production. However, the mechanisms underlying the male sterility induced by SQ-1 still remain poorly understood. In this study, a Cyclin-Dependent Kinase Inhibitor gene, <i>TaICK1</i>, which encodes a 229 amino acid protein, was identified as a potential contributor to male sterility in common wheat. The expression of <i>TaICK1</i> was upregulated during the development of anthers in Xinong1376 wheat treated with SQ-1. Meanwhile, the seed setting rate was found to be significantly decreased in <i>TaICK1</i> transgenic rice. Furthermore, we identified two cyclin proteins, <i>TaCYCD2;1</i> and <i>TaCYCD6;1</i>, as interactors through yeast two-hybrid screening using TaICK1 as the bait, which were validated using bimolecular fluorescence complementation. Subcellular localization revealed that the proteins encoded by <i>TaICK1</i>, <i>TaCYCD2;1,</i> and <i>TaCYCD6;1</i> were localized in the cell nucleus. The expression levels of <i>TaCYCD2;1</i> and <i>TaCYCD6;1</i> were lower in Xinong1376 treated with SQ-1. A further analysis demonstrated that the expression levels of <i>OsCYCD2;1</i> and <i>OsCYCD6;1</i> were lower in transgenic <i>TaICK1</i> rice lines as well. Taken together, these results suggest that the upregulation of <i>TaICK1,</i> induced by SQ-1, may subsequently suppress the expression of <i>TaCYCD2;1</i> and <i>TaCYCD6;1</i> in anthers, resulting in male sterility. This study provides new insights into the understanding of SQ-1-induced wheat male sterility, as well as the developmental mechanisms of anthers. |
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| Journal | Int J Mol Sci | |
| Country | China | |
| Volume | 21(7) | |
| Pages | ||
| Year | 2020 | |
| PubMed ID | 32252420 | |
| PubMed Central ID | 7177297 | |
| DOI | 10.3390/ijms21072468 | |
| URL | - | |
| Relation | ||
| Gene | CYCD2;2 CYCD6;1 KRP1 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
