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Reference Detail
| Reference | ||
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| Author | Seo H., Kim S.H., Lee B.D., Lim J.H., Lee S.J., An G., Paek N.C. | |
| Title | The Rice <i>Basic Helix-Loop-Helix 79</i> (<i>OsbHLH079</i>) Determines leaf angle and Grain Shape. | |
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Abstract: Changes in plant architecture, such as leaf size, leaf shape, leaf angle, plant height, and floral organs, have been major factors in improving the yield of cereal crops. Moreover, changes in grain size and weight can also increase yield. Therefore, screens for additional factors affecting plant architecture and grain morphology may enable additional improvements in yield. Among the basic Helix-Loop-Helix (bHLH) transcription factors in rice (<i>Oryza sativa</i>), we found an enhancer-trap T-DNA insertion mutant of <i>OsbHLH079</i> (termed <i>OsbHLH079-D</i>). The <i>OsbHLH079-D</i> mutant showed a wide leaf angle phenotype and produced long grains, similar to the phenotypes of mutants with increased brassinosteroid (BR) levels or enhanced BR signaling. Reverse transcription-quantitative PCR analysis showed that BR signaling-associated genes are largely upregulated in <i>OsbHLH079-D</i>, but BR biosynthesis-associated genes are not upregulated, compared with its parental <i>japonica</i> cultivar 'Dongjin'. Consistent with this, <i>OsbHLH079-D</i> was hypersensitive to BR treatment. Scanning electron microscopy revealed that the expansion of cell size in the adaxial side of the lamina joint was responsible for the increase in leaf angle in <i>OsbHLH079-D</i>. The expression of cell-elongation-associated genes encoding expansins and xyloglucan endotransglycosylases/hydrolases increased in the lamina joints of leaves in <i>OsbHLH079-D</i>. The regulatory function of OsbHLH079 was further confirmed by analyzing <i>35S::OsbHLH079</i> overexpression and <i>35S::RNAi-OsbHLH079</i> gene silencing lines. The <i>35S::OsbHLH079</i> plants showed similar phenotypes to <i>OsbHLH079-D</i>, and the <i>35S::RNAi-OsbHLH079</i> plants displayed opposite phenotypes to <i>OsbHLH079-D</i>. Taking these observations together, we propose that <i>OsbHLH079</i> functions as a positive regulator of BR signaling in rice. |
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| Journal | Int J Mol Sci | |
| Country | South Korea | |
| Volume | 21(6) | |
| Pages | ||
| Year | 2020 | |
| PubMed ID | 32197452 | |
| PubMed Central ID | 7139501 | |
| DOI | 10.3390/ijms21062090 | |
| URL | - | |
| Relation | ||
| Gene | BIN2 BLR1 BRD1 BU1 BZR1 D11 D2 DLT EXPA3 EXPA4 EXPA5 EXPA6 EXPA7 GW2 ILI1 PIL13 RLCK173 SG2 TGW6 XTH2 XTH28 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
