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Reference Detail
| Reference | ||
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| Author | Hu L., Tu B., Yang W., Yuan H., Li J., Guo L., Zheng L., Chen W., Zhu X., Wang Y., Qin P., Ma B., Li S. | |
| Title | Mitochondria-Associated pyruvate kinase Complexes Regulate Grain Filling in Rice. | |
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Abstract: Grain filling is a complex agronomic trait that directly determines grain weight and quality in rice (<i>Oryza sativa</i>). Nevertheless, key factors affecting grain filling remain poorly understood. Here, we identified a grain filling gene, <i>OsPK3</i>, encoding a pyruvate kinase (PK). The loss of function of <i>OsPK3</i> caused reduced PK activity and Suc translocation defects from source to sink in rice, which led to compromised grain filling. <i>OsPK3</i> was constitutively expressed but had relatively higher expression levels in leaf and developing caryopsis and specific expression signals in tissues involved in Suc transport and unloading, supporting its biological function in regulation of grain filling by affecting Suc translocation. Subcellular localization analysis of OsPK3 revealed its association with mitochondria, and OsPK3 physically interacted and formed heterodimers in vivo with two other PK isozymes, OsPK1 and OsPK4. Both OsPK1 and OsPK4 localized to the mitochondria and cytosol and were recruited to the mitochondria by OsPK3. Despite their high sequence similarity, OsPK1 and OsPK4 had distinct expression patterns. As observed for <i>OsPK3</i>, disruption of <i>OsPK1</i> caused pleiotropic defects, while <i>OsPK4</i> loss of function led to severely chalky grains without other obvious defects. Collectively, we revealed that two mitochondria-associated pyruvate kinase complexes, OsPK3-OsPK1/OsPK4, are involved in regulation of grain filling by stage-specific fine-tuning of Suc translocation. |
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| Journal | Plant Physiol. | |
| Country | China | |
| Volume | 183(3) | |
| Pages | 1073-1087 | |
| Year | 2020 | |
| PubMed ID | 32376763 | |
| PubMed Central ID | - | |
| DOI | 10.1104/pp.20.00279 | |
| URL | - | |
| Relation | ||
| Gene | AGPL2 AGPS2 ALK BT1-1 GBSSII GIF1 PHO1 PK1 PK3 PK4 SSI SUT1 SUT4 SUT5 SWEET15 WX1 XA13 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
