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| 文献 | ||
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| 著者 | Liang J., He Y., Zhang Q., Wang W., Zhang Z. | |
| タイトル | Plasma Membrane Ca2+ Permeable Mechanosensitive Channel <i>Osdmt1</i> Is Involved in Regulation of Plant Architecture and Ion Homeostasis in Rice. | |
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Abstract: Plant architecture is an important factor for crop production. Plant height, tiller pattern, and panicle morphology are decisive factors for high grain yield in rice. Here, we isolated and characterized a T-DNA insertion rice mutant <i>Osdmt1</i> (Oryza sativa dwarf and multi-tillering1) that exhibited a severe dwarf phenotype and multi-tillering. Molecular cloning revealed that <i>DMT1</i> encodes a plasma membrane protein that was identified as a putative Ca2+ permeable mechanosensitive channel. The transcript expression level was significantly higher in the <i>dmt1</i> mutant compared to wild type (WT). Additionally, the <i>dmt1</i> homozygous mutant displayed a stronger phenotype than that of the WT and heterozygous seedlings after gibberellic acid (GA) treatment. RNA-seq and iTRAQ-based proteome analyses were performed between the <i>dmt1</i> mutant and WT. The transcriptome profile revealed that several genes involved in GA and strigolactone (SL) biosyntheses were altered in the <i>dmt1</i> mutant. Ca2+ and other ion concentrations were significantly enhanced in the <i>dmt1</i> mutant, suggesting that <i>DMT1</i> contributes to the accumulation of several ions in rice. Moreover, several EF-hand Ca2+ sensors, including CMLs (CaM-like proteins) and CDPKs (calcium-dependent protein kinases), displayed markedly altered transcript expression and protein levels in the <i>dmt1</i> mutant. Overall, these findings aid in the elucidation of the multiply regulatory roles of <i>Osdmt1/OsMCA1</i> in rice. |
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| 掲載誌 | Int J Mol Sci | |
| Country | China | |
| 刊数 | 21(3) | |
| ページ | ||
| 発刊年 | 2020 | |
| PubMed ID | 32046032 | |
| PubMed Central ID | 7037369 | |
| DOI | 10.3390/ijms21031097 | |
| URL | - | |
| 関連情報 | ||
| 遺伝子 | CML11 CML15 CML16 CML2 CPS4 D14 D17 DMT1 KS7 | |
| INSD | - | |
| 系統 |
野生イネ コアコレクション |
- |
| 突然変異系統(遺伝研 Collection) | - | |
| 種子不稔系統 | - | |
| 致死変異体系統 | - | |
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組織別発生段階 - 突然変異系統(遺伝子) |
- | |
| 栽培イネ品種(遺伝研 Collection) | - | |
| 組織別発生段階 | - | |
