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Reference Detail
| Reference | ||
|---|---|---|
| Author | Sun L., Di D.W., Li G., Kronzucker H.J., Wu X., Shi W. | |
| Title | Endogenous ABA Alleviates Rice Ammonium Toxicity by Reducing ROS and Free Ammonium via Regulation of the SAPK9-bZIP20 Pathway. | |
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Abstract: Ammonium (NH4+) is one of the principal nitrogen (N) sources in soils but is typically toxic already at intermediate concentrations. The phytohormone abscisic acid (ABA) plays a pivotal role in responses to environmental stresses. However, the role of ABA under high-NH4+ stress in rice (Oryza sativa L.) is only marginally understood. Here, we report that elevated NH4+ can significantly accelerate tissue ABA accumulation. Mutants with high (Osaba8ox) and low levels of ABA (Osphs3-1) exhibit elevated tolerance or sensitivity to high-NH4+ stress, respectively. Furthermore, ABA can decrease NH4+-induced oxidative damage and tissue NH4+ accumulation by enhancing antioxidant and GS/GOGAT enzyme activities. Using RNA-seq and qRT-PCR approaches, we ascertain that two genes, OsSAPK9 and OsbZIP20, are induced by both high NH4+ and by ABA. Our data indicate that OsSAPK9 interacts with OsbZIP20, and can phosphorylate OsbZIP20 and activate its function. When OsSAPK9 or OsbZIP20 are knocked out in rice, ABA-mediated antioxidant and GS/GOGAT activity enhancement under high-NH4+ stress disappear, and the two mutants are more sensitive to high-NH4+ stress compared with their wild types. Taken together, our results suggest that ABA plays a positive role in regulating the OsSAPK9-OsbZIP20 pathway in rice to increase tolerance to high-NH4+ stress. |
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| Journal | J. Exp. Bot. | |
| Country | China | |
| Volume | ||
| Pages | ||
| Year | 2020 | |
| PubMed ID | 32064504 | |
| PubMed Central ID | - | |
| DOI | 10.1093/jxb/eraa076 | |
| URL | - | |
| Relation | ||
| Gene | ABA8OX3 PHS3 RITA1 SAPK9 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
