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Reference Detail
| Reference | ||
|---|---|---|
| Author | Jia Y., Li C., Li Q., Liu P., Liu D., Liu Z., Wang Y., Jiang G., Zhai W. | |
| Title | Characteristic Dissection of <i>Xanthomonas oryzae</i> pv. o<i>ryzae</i> Responsive MicroRNAs in Rice. | |
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Abstract: MicroRNAs (miRNAs) are crucial player in plant-pathogen interaction. While the evidence has demonstrated that rice miRNAs mediate immune response to pathogens invasion, the roles of miRNAs on <i>Xanthomonas oryzae</i> pv. <i>oryzae</i> (<i>Xoo</i>) attack remain be in place. Herein, we monitored the responsive changes of rice miRNAs at 0, 8, 24 h across <i>Xoo</i> strain PXO86 infection in its compatible rice variety IR24 and incompatible variety IRBB5 by small RNA sequencing, and the genes targeted by miRNAs were also detected via degradome technology. The faithfulness of sequencing data was validated through quantitative real-time stem-loop reverse transcription-polymerase chain reaction assay. Bioinformatic analysis showed that the differentially expressed miRNAs could be divided into three immunity-related clusters, and 80 regulatory units were emerged in infection process, which comprises 29 differentially expressed known miRNAs and 38 cleaved targets. Furthermore, the miRNA presumptive function of separate immunity cluster in rice-Xoo interplay was confirmed through overexpressing osa-miR164a, osa-miR167d and osa-miR159b, and the disruption of regulatory units, osa-miR164a/<i>OsNAC60,</i> osa-miR167d-5p/<i>OsWD40-174</i> and osa-miR159b/<i>OsMYBGA</i>, <i>OsLRR-RLK2</i>, <i>OsMPK20-4</i>, may reset rice defense response to <i>Xoo</i> infestation in a controllable manner<i>.</i> These findings provide new insights into the complex roles of characteristic miRNAs and their targets in rice-<i>Xoo</i> interactions. |
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| Journal | Int J Mol Sci | |
| Country | China | |
| Volume | 21(3) | |
| Pages | ||
| Year | 2020 | |
| PubMed ID | 31991765 | |
| PubMed Central ID | 7037501 | |
| DOI | 10.3390/ijms21030785 | |
| URL | - | |
| Relation | ||
| Gene | AGO1A AGO1B DCL1 GAMYB GF14C HUB2 LSD3 MIR1432 MIR160A MIR164A MIR167A MIR167D MIR528 NAC60 ORAP1 RIR1 RMT1 SIP31 UGT83A1 WJUMK1 _ _ miR159B | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
