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Reference Detail
| Reference | ||
|---|---|---|
| Author | Sakuraba Y., Kim D., Han S.H., Kim S.H., Piao W., Yanagisawa S., An G., Paek N.C. | |
| Title | Multilayered Regulation of Membrane-Bound ONAC054 Is Essential for Abscisic Acid-Induced leaf senescence in Rice. | |
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Abstract: In most plants, abscisic acid (ABA) induces premature leaf senescence; however, the mechanisms of ABA signaling during leaf senescence remain largely unknown. Here, we show that the rice (<i>Oryza sativa</i>) NAM/ATAF1/2/CUC2 (NAC) transcription factor ONAC054 plays an important role in ABA-induced leaf senescence. The <i>ONAC054</i> knockout mutants maintained green leaves, while <i>ONAC054</i>-overexpressing lines showed early leaf yellowing under dark- and ABA-induced senescence conditions. Genome-wide microarray analysis showed that ABA signaling-associated genes, including <i>ABA INSENSITIVE5</i> (<i>OsABI5</i>) and senescence-associated genes, including <i>STAY-GREEN</i> and <i>NON-YELLOW COLORING1</i> (<i>NYC1</i>), were significantly down-regulated in <i>ONAC054</i> mutants. Chromatin immunoprecipitation and protoplast transient assays showed that ONAC054 directly activates <i>OsABI5</i> and <i>NYC1</i> by binding to the mitochondrial dysfunction motif in their promoters. ONAC054 activity is regulated by proteolytic processing of the C-terminal transmembrane domain (TMD). We found that nuclear import of ONAC054 requires cleavage of the putative C-terminal TMD. Furthermore, the <i>ONAC054</i> transcript (termed <i>ONAC054alpha</i>) has an alternatively spliced form (<i>ONAC054beta</i>), with seven nucleotides inserted between intron 5 and exon 6, truncating ONAC054alpha protein at a premature stop codon. ONAC054beta lacks the TMD and thus localizes to the nucleus. These findings demonstrate that the activity of ONAC054, which is important for ABA-induced leaf senescence in rice, is precisely controlled by multilayered regulatory processes. |
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| Journal | Plant Cell | |
| Country | South Korea | |
| Volume | 32(3) | |
| Pages | 630-649 | |
| Year | 2020 | |
| PubMed ID | 31911455 | |
| PubMed Central ID | 7054035 | |
| DOI | 10.1105/tpc.19.00569 | |
| URL | - | |
| Relation | ||
| Gene | ABI5 BZIP23 BZIP72 NAC1 NAC58 NOL NYC1 RIM1 SGR TAC5 TIL1 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
