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Reference Detail
| Reference | ||
|---|---|---|
| Author | Jiao X., Wang H., Yan J., Kong X., Liu Y., Chu J., Chen X., Fang R., Yan Y. | |
| Title | Promotion of BR Biosynthesis by miR444 Is Required for Ammonium-Triggered Inhibition of Root Growth. | |
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Abstract: Rice (<i>Oryza sativa</i>), the staple food for almost half of the world's population, prefers ammonium (NH4+) as the major nitrogen resource, and while NH4+ has profound effects on rice growth and yields, the underlying regulatory mechanisms remain largely unknown. Brassinosteroids (BRs) are a class of steroidal hormones playing key roles in plant growth and development. In this study, we show that NH4+ promotes BR biosynthesis through miR444 to regulate rice root growth. miR444 targeted five homologous MADS-box transcription repressors potentially forming homologous or heterogeneous complexes in rice. miR444 positively regulated BR biosynthesis through its MADS-box targets, which directly repress the transcription of <i>BR-deficient dwarf 1</i> (<i>OsBRD1</i>), a key BR biosynthetic gene. NH4+ induced the miR444-OsBRD1 signaling cascade in roots, thereby increasing the amount of BRs, whose biosynthesis and signaling were required for NH4+<b>-</b>dependent root elongation inhibition. Consistently, miR444-overexpressing rice roots were hypersensitive to NH4+ depending on BR biosynthesis, and overexpression of miR444's target, <i>OsMADS57</i>, resulted in rice hyposensitivity to NH4+ in root elongation, which was associated with a reduction of BR content. In summary, our findings reveal a cross talk mechanism between NH4+ and BR in which NH4+ activates miR444-OsBRD1, an undescribed BR biosynthesis-promoting signaling cascade, to increase BR content, inhibiting root elongation in rice. |
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| Journal | Plant Physiol. | |
| Country | China | |
| Volume | 182(3) | |
| Pages | 1454-1466 | |
| Year | 2020 | |
| PubMed ID | 31871071 | |
| PubMed Central ID | 7054888 | |
| DOI | 10.1104/pp.19.00190 | |
| URL | - | |
| Relation | ||
| Gene | BRD1 BRD2 BZR1 D11 D61 DWARF4 MADS23 MADS25 MADS27 MADS57 MADS61 MIR444A MIR444B MIR444C MIR444D MIR444E MIR444F | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
