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Reference Detail
| Reference | ||
|---|---|---|
| Author | Xue M., Liu L., Yu Y., Zhu J., Gao H., Wang Y., Wan J. | |
| Title | Lose-of-Function of a Rice Nucleolus-Localized Pentatricopeptide Repeat Protein Is Responsible for the floury endosperm14 Mutant Phenotypes. | |
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Abstract: The endosperm of rice (Oryza sativa) has been usually used for the study of starch synthesis. Although several related factors have been revealed, other unknown members remain to be identified, given that starch synthesis is a complicated and sophisticated process. Here, we identified and characterized a new rice seed mutant, floury endosperm14 (FLO14), which showed chalked endosperm and seed-lethal phenotypes. Map-based cloning indicated FLO14 encodes a novel P-family PPR protein which contains ten PPR motifs. Afterwards the gene was named OsNPPR3. Subcellular localization showed OsNPPR3 was targeted to nucleolus. Quantitative RT-PCR analysis demonstrated that OsNPPR3 was universally expressed in various tissues, with pronounced levels during rice endosperm development. Molecular analysis further suggested that OsNPPR3 was involved in the regulation of expression levels and splicing of a few genes in mitochondria. The study demonstrates that the nucleolus-localized PPR protein is responsible for the FLO14 mutant phenotypes through affecting nuclear and mitochondrial gene expression and splicing. |
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| Journal | Rice (N Y) | |
| Country | China | |
| Volume | 12(1) | |
| Pages | 100 | |
| Year | 2019 | |
| PubMed ID | 31889223 | |
| PubMed Central ID | 6937366 | |
| DOI | 10.1186/s12284-019-0359-x | |
| URL | - | |
| Relation | ||
| Gene | AGPS2 ALK FLO14 ISA2 PHO1 PHS8 RPS2 SBE3 _ _ | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
|
Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
