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Reference Detail
| Reference | ||
|---|---|---|
| Author | Xiong Y., Ren Y., Li W., Wu F., Yang W., Huang X., Yao J. | |
| Title | NF-YC12 is a key multi-functional regulator of accumulation of seed storage substances in rice. | |
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Abstract: Starch and storage proteins, the primary storage substances of cereal endosperm, are a major source of food for humans. However, the transcriptional regulatory networks of the synthesis and accumulation of storage substances remain largely unknown. Here, we identified a rice endosperm-specific gene, NF-YC12, that encodes a putative nuclear factor-Y transcription factor subunit C. NF-YC12 is expressed in the aleurone layer and starchy endosperm during grain development. Knockout of NF-YC12 significantly decreased grain weight as well as altering starch and protein accumulation and starch granule formation. RNA-sequencing analysis revealed that in the NF-YC12 mutant genes related to starch biosynthesis and the metabolism of energy reserves were enriched in the down-regulated category. In addition, starch and protein contents in seeds differed between NF-YC12-overexpression lines and the wild-type. NF-YC12 was found to interact with NF-YB1. ChIP-qPCR and yeast one-hybrid assays showed that NF-YC12 regulated the rice sucrose transporter OsSUT1 in coordination with NF-YB1 in the aleurone layer. In addition, NF-YC12 was directly bound to the promoters of FLO6 (FLOURY ENDOSPERM6) and OsGS1;3 (glutamine synthetase1) in developing endosperm. This study demonstrates a transcriptional regulatory network involving NF-YC12, which coordinates multiple pathways to regulate endosperm development and the accumulation of storage substances in rice seeds. |
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| Journal | J. Exp. Bot. | |
| Country | China | |
| Volume | 70(15) | |
| Pages | 3765-3780 | |
| Year | 2019 | |
| PubMed ID | 31211389 | |
| PubMed Central ID | 6685661 | |
| DOI | 10.1093/jxb/erz168 | |
| URL | - | |
| Relation | ||
| Gene | AGPL2 AGPS1 AGPS2 FLO5 FLO6 GLN1;3 GLUB1 GLUD1 HAP3K HAP5L HXK7 MST6 SSIIIB SUT1 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
