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Reference Detail
| Reference | ||
|---|---|---|
| Author | Zhao K., Lin F., Romero-Gamboa SP., Saha P., Goh H.J., An G., Jung K.H., Hazen S.P., Bartley L.E. | |
| Title | Rice Genome-Scale Network Integration Reveals Transcriptional Regulators of Grass Cell Wall Synthesis. | |
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Abstract: Grasses have evolved distinct cell wall composition and patterning relative to dicotyledonous plants. However, despite the importance of this plant family, transcriptional regulation of its cell wall biosynthesis is poorly understood. To identify grass cell wall-associated transcription factors, we constructed the Rice Combined mutual Ranked Network (RCRN). The RCRN covers >90% of annotated rice (<i>Oryza sativa</i>) genes, is high quality, and includes most grass-specific cell wall genes, such as mixed-linkage glucan synthases and hydroxycinnamoyl acyltransferases. Comparing the RCRN and an equivalent <i>Arabidopsis</i> network suggests that grass orthologs of most genetically verified eudicot cell wall regulators also control this process in grasses, but some transcription factors vary significantly in network connectivity between these divergent species. Reverse genetics, yeast-one-hybrid, and protoplast-based assays reveal that OsMYB61a activates a grass-specific acyltransferase promoter, which confirms network predictions and supports grass-specific cell wall synthesis genes being incorporated into conserved regulatory circuits. In addition, 10 of 15 tested transcription factors, including six novel Wall-Associated regulators (WAP1, WACH1, WAHL1, WADH1, OsMYB13a, and OsMYB13b), alter abundance of cell wall-related transcripts when transiently expressed. The results highlight the quality of the RCRN for examining rice biology, provide insight into the evolution of cell wall regulation, and identify network nodes and edges that are possible leads for improving cell wall composition. |
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| Journal | Front Plant Sci | |
| Country | USA | |
| Volume | 10 | |
| Pages | 1275 | |
| Year | 2019 | |
| PubMed ID | 31681374 | |
| PubMed Central ID | 6813959 | |
| DOI | 10.3389/fpls.2019.01275 | |
| URL | - | |
| Relation | ||
| Gene | 4CL3 AT1 AT5 AT6 BC6 BC7 CEF1 CSLF6 CSLH1 DLN107 F5HL2 GH2 LG3 MYB30 NAC73 PMT ROMT9 RRS1 WAHD1 _ _ _ _ _ _ | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
