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Reference Detail
| Reference | ||
|---|---|---|
| Author | Zhang Q., Shen L., Wang Z., Hu G., Ren D., Hu J., Zhu L., Gao Z., Zhang G., Guo L., Zeng D., Qian Q. | |
| Title | OsCAF1, a CRM Domain Containing Protein, Influences Chloroplast Development. | |
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Abstract: The chloroplast RNA splicing and ribosome maturation (CRM) domain proteins are involved in the splicing of chloroplast gene introns. Numerous CRM domain proteins have been reported to play key roles in chloroplast development in several plant species. However, the functions of CRM domain proteins in chloroplast development in rice remain poorly understood. In the study, we generated <i>OsCAF1</i> albino mutants, which eventually died at the seedling stage, through the editing of <i>OsCAF1</i> with two CRM domains using CRISPR/Cas9 technology. The mesophyll cells in <i>OsCAF1</i> mutant had decreased chloroplast numbers and damaged chloroplast structures. OsCAF1 was located in the chloroplast, and transcripts revealed high levels in green tissues. In addition, the OsCAF1 promoted the splicing of group IIA and group IIB introns, unlike orthologous proteins of AtCAF1 and ZmCAF1, which only affected the splicing of subgroup IIB introns. We also observed that the C-terminal of OsCAF1 interacts with OsCRS2, and OsCAF1-OsCRS2 complex may participate in the splicing of group IIA and group IIB introns in rice chloroplasts. OsCAF1 regulates chloroplast development by influencing the splicing of group II introns. |
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| Journal | Int J Mol Sci | |
| Country | China | |
| Volume | 20(18) | |
| Pages | ||
| Year | 2019 | |
| PubMed ID | 31500108 | |
| PubMed Central ID | 6770308 | |
| DOI | 10.3390/ijms20184386 | |
| URL | - | |
| Relation | ||
| Gene | APX1 APX2 CAB1R CAF1 CATA CHLH CRS2 ELL FGL HSA1 LSD3 NOE1 PSAA PSBA RBCL RPOB RPOC1 RPOC2 SODA1 SODB WLP2 WP2 YGL1 _ _ _ | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
