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Author Mao C., He J., Liu L., Deng Q., Yao X., Liu C., Qiao Y., Li P., Ming F.
Title OsNAC2 integrates auxin and cytokinin pathways to modulate rice root development.
Abstract:
The rice root system is important for growth. The crosstalk between auxin and cytokinin mediates root initiation and elongation. However, it remains unclear how the transcriptional network upstream of the auxin and cytokinin signalling pathways determines root development. Here, we observed that the knockdown of OsNAC2, which encodes a NAC transcription factor, increased the primary root length and the number of crown roots. OsNAC2 predominantly expressed in primary root tips, crown roots and lateral root primordia, implying it influences root development. Molecular analyses revealed that the expressions of auxin- and cytokinin-responsive genes were affected in OsNAC2-overexpressing (OsNAC2-OX; ON7 and ON11), RNA interference (OsNAC2-RNAi; RNAi25 and RNAi31) and CRISPR/Cas9 plants. Additionally, OsNAC2 can directly bind to the promoters of IAA inactivation-related genes (GH3.6 and GH3.8), an IAA signalling-related gene (OsARF25), and a cytokinin oxidase gene (OsCKX4). Furthermore, genetic analysis of ON11/OsGH3.6 and RNAi31/OsCKX4 homozygote confirmed that OsCKX4 and OsGH3.6 functioned downstream of OsNAC2. The mRNA levels of CROWN ROOTLESS (CRL) genes and cyclin-dependent protein kinase (CDK) genes increased in OsNAC2-RNAi and OsNAC2-Cas9 lines while reduced in OsNAC2-OX lines. Thus, we describe that OsNAC2 functions as an upstream integrator of auxin and cytokinin signals that affect CRL and CDK production to regulate cell division during root development. This novel auxin-OsNAC2-cytokinin model should provide a new insight into the understanding of NAC TFs and crosstalk of auxin and cytokinin pathway, and can be potentially applied in agriculture to enhance rice yields by genetic approaches.
Journal Plant Biotechnol. J.
Country China
Volume 18(2)
Pages 429-442
Year 2020
PubMed ID 31389120
PubMed Central ID 6953191
DOI 10.1111/pbi.13209
URL -
Relation
Gene ARF6B CDC2-1 CDC2-2 CDKB;1 CKX4 CKX5 CRL1 CRL4 GH3-6 GH3-8 HST1 IAA31 IAA9 IPT3 IPT5 LC1 LOGL3 NAC2 PLT8 RR1 RR11 RR23 RR3 RR4 WOX11 YUCCA5 YUCCA6
INSD -
Strain Wild Core Collection -
Induced Mutation Lines(NIG Collection) -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain
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Stages in Each Organ
- Muant Lines (Gene)
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Cultivated Varieties(NIG Collection) -
Stages in Each Organ -
/rice/oryzabase