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Author Jiang M., Liu Y., Li R., Zheng Y., Fu H., Tan Y., Møller IM., Fan L., Shu Q., Huang J.
Title A Suppressor Mutation Partially Reverts the <i>xantha</i> Trait <i>via</i> Lowered Methylation in the Promoter of <i>Genomes Uncoupled 4</i> in Rice.
Abstract:
The <i>xantha</i> trait of a yellow leaf rice mutant (HYB), controlled epigenetically by elevated CHG methylation of the <i>genomes uncoupled 4</i> (<i>OsGUN4</i>) promoter, has reduced chlorophyll content, altered tetrapyrrole biosynthesis, and deregulated transcription of photosynthesis-associated nuclear genes (PhANGs) compared to its wild-type progenitor Longtefu B (LTB). In the present study, we identified a suppressor mutant (CYB) of HYB and characterized its genetic, molecular, and physiological basis of the mutant phenotype. We found that the light-green phenotype of CYB was caused by a suppressor mutation in an unknown gene other than <i>OsGUN4</i>. Compared to HYB, the CHG methylation in the <i>OsGUN4</i> promoter was reduced, while OsGUN4 transcript and protein abundance levels were increased in CYB. The contents of total chlorophyll and its intermediate metabolites (except protoporphyrin IX) in CYB plants were intermediate between HYB and LTB. The expression levels of 30 genes involved in tetrapyrrole biosynthesis in CYB were all partially reverted to those of LTB, so were the PhANGs. In summary, a suppressor mutation caused the reversion of the <i>xantha</i> trait <i>via</i> reducing CHG methylation in <i>OsGUN4</i> promoter.
Journal Front Plant Sci
Country China
Volume 10
Pages 1003
Year 2019
PubMed ID 31428119
PubMed Central ID 6688194
DOI 10.3389/fpls.2019.01003
URL -
Relation
Gene GUN4
INSD -
Strain Wild Core Collection -
Induced Mutation Lines(NIG Collection) -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain 		-
Stages in Each Organ
- Muant Lines (Gene) 		-
Cultivated Varieties(NIG Collection) -
Stages in Each Organ -
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