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Reference Detail
| Reference | ||
|---|---|---|
| Author | Wang H., Zhang Y., Sun L., Xu P., Tu R., Meng S., Wu W., Anis G.B., Hussain K., Riaz A., Chen D., Cao L., Cheng S., Shen X. | |
| Title | <i>WB1</i>, a Regulator of Endosperm Development in Rice, Is Identified by a Modified MutMap Method. | |
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Abstract: Abnormally developed endosperm strongly affects rice (<i>Oryza sativa</i>) appearance quality and grain weight. Endosperm formation is a complex process, and although many enzymes and related regulators have been identified, many other related factors remain largely unknown. Here, we report the isolation and characterization of a recessive mutation of <i>White Belly 1</i> (<i>WB1</i>), which regulates rice endosperm development, using a modified MutMap method in the rice mutant <i>wb1</i>. The <i>wb1</i> mutant develops a white-belly endosperm and abnormal starch granules in the inner portion of white grains. Representative of the white-belly phenotype, grains of <i>wb1</i> showed a higher grain chalkiness rate and degree and a lower 1000-grain weight (decreased by ~34%), in comparison with that of Wild Type (WT). The contents of amylose and amylopectin in <i>wb1</i> significantly decreased, and its physical properties were also altered. We adopted the modified MutMap method to identify 2.52 Mb candidate regions with a high specificity, where we detected 275 SNPs in chromosome 4. Finally, we identified 19 SNPs at 12 candidate genes. Transcript levels analysis of all candidate genes showed that <i>WB1</i> (<i>Os04t0413500</i>), encoding a cell-wall invertase, was the most probable cause of white-belly endosperm phenotype. Switching off <i>WB1</i> with the CRISPR/cas9 system in Japonica cv. Nipponbare demonstrates that <i>WB1</i> regulates endosperm development and that different mutations of <i>WB1</i> disrupt its biological function. All of these results taken together suggest that the <i>wb1</i> mutant is controlled by the mutation of <i>WB1</i>, and that the modified MutMap method is feasible to identify mutant genes, and could promote genetic improvement in rice. |
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| Journal | Int J Mol Sci | |
| Country | China | |
| Volume | 19(8) | |
| Pages | ||
| Year | 2018 | |
| PubMed ID | 30042352 | |
| PubMed Central ID | 6121324 | |
| DOI | 10.3390/ijms19082159 | |
| URL | - | |
| Relation | ||
| Gene | AGPL1 AGPS1 FLO4 FLO6 GIF1 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
