Update info.Tools - Reference - Detail
Reference Detail
| Reference | ||
|---|---|---|
| Author | Bai W., Wang P., Hong J., Kong W., Xiao Y., Yu X., Zheng H., You S., Lu J., Lei D., Wang C., Wang Q., Liu S., Liu X., Tian Y., Chen L., Jiang L., Zhao Z., Wu C., Wan J. | |
| Title | <i>Earlier Degraded Tapetum1</i> (<i>EDT1</i>) Encodes an ATP-citrate lyase Required for Tapetum Programmed Cell Death. | |
|
Abstract: In flowering plants, the tapetum cells in anthers undergo programmed cell death (PCD) at the late meiotic stage, providing nutrients for further development of microspores, including the formation of the pollen wall. However, the molecular basis of tapetum PCD remains elusive. Here we report a tapetum PCD-related mutant in rice (<i>Oryza sativa</i>), <i>earlier degraded tapetum 1</i> (<i>edt1</i>), that shows complete pollen abortion associated with earlier-than-programmed tapetum cell death. <i>EDT1</i> encodes a subunit of ATP-citrate lyase (ACL), and is specifically expressed in the tapetum of anthers. EDT1 localized in both the nucleus and the cytoplasm as observed in rice protoplast transient assays. We demonstrated that the A and B subunits of ACL interacted with each other and might function as a heteromultimer in the cytoplasm. EDT1 catalyzes the critical steps in cytosolic acetyl-CoA synthesis. Our data indicated a decrease in ATP level, energy charge, and fatty acid content in mutant <i>edt1</i> anthers. In addition, the genes encoding secretory proteases or lipid transporters, and the transcription factors known to regulate PCD, were downregulated. Our results demonstrate that the timing of tapetum PCD must be tightly regulated for successful pollen development, and that <i>EDT1</i> is involved in the tapetum PCD process. This study furthers our understanding of the molecular basis of pollen fertility and fecundity in rice and may also be relevant to other flowering plants. |
||
| Journal | Plant Physiol. | |
| Country | China | |
| Volume | 181(3) | |
| Pages | 1223-1238 | |
| Year | 2019 | |
| PubMed ID | 31515447 | |
| PubMed Central ID | 6836821 | |
| DOI | 10.1104/pp.19.00202 | |
| URL | - | |
| Relation | ||
| Gene | CP1 CYP704B2 EAT1 EDT1 MS2 RADC1 TDR | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
|
Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
