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| 文献 | ||
|---|---|---|
| 著者 | Atul Prakash Sathe, Su X., Chen Z., Chen T., Wei X., Tang S., Xiao-Bo Zhang, Jian-Li Wu | |
| タイトル | Identification and characterization of a spotted-leaf mutant spl40 with enhanced bacterial blight resistance in rice. | |
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Abstract: Spotted leaf mutants show typical necrotic lesions that appear spontaneously in the absence of any pathogen attack. These mutants are often characterized to exhibit programmed cell death (PCD) and activation of plant defense responses resulting in enhanced disease resistance to multiple pathogens. Here, we reported a novel spotted-leaf mutant, spl40 that showed enhanced disease resistance response. Initially lesions appeared at leaf tips during seedling stage and gradually covered the whole leaf at the tillering stage. The lesion development was light-dependent. spl40 showed obvious cell death at and around the lesion, and burst of reactive oxygen species (ROS) was accompanied by disturbed ROS scavenging system. Photosynthetic capacity was compromised as evidenced by significant reductions in chlorophyll content, important photosynthesis parameters and downregulated expression of photosynthesis-related genes which ultimately led to poor performance of major agronomic traits. spl40 exhibited enhanced resistance to 14 out of 16 races of bacterial blight pathogen of rice, caused by Xanthomonas oryzae pv. oryzae, most probably though activation of SA and JA signaling pathways, owing to upregulated expression of SA and JA signaling genes, though the exact mechanism remain to be elucidated. The spotted-leaf phenotype was controlled by a novel single recessive nuclear gene. Genetic mapping combined with high throughput sequencing analysis identified Os05G0312000 as the most probable candidate gene. Sequencing of ORF revealed a single SNP change from C to T that resulted in non-synonymous change in amino acid residue from leucine to phenylalanine. Interestingly, the complementation plants did not display lesions before heading but showed lesions at the heading stage and the transgenic T1 progenies could be classified into 3 categories based on their lesion intensity, indicating the complex genetic nature of the spl40 mutation. The results obtained here clearly show that genes related to defense and PCD were upregulated in accordance with enhanced disease resistance and occurrence of PCD, whereas the photosynthetic capacity and overall ROS homeostasis was compromised in spl40. Our data suggest that a novel spotted-leaf mutant, spl40, would help to elucidate the mechanism behind lesion development involving programmed cell death and associated defense responses. |
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| 掲載誌 | Rice (N Y) | |
| Country | China | |
| 刊数 | 12(1) | |
| ページ | 68 | |
| 発刊年 | 2019 | |
| PubMed ID | 31446514 | |
| PubMed Central ID | 6708518 | |
| DOI | 10.1186/s12284-019-0326-6 | |
| URL | - | |
| 関連情報 | ||
| 遺伝子 | AMTR1 AOS2 CHS CHT4 ELL FGL HEMA KAT1 MC2 MC3 MC4 MC5 MC6 MC8 NH1 NOE1 PAL PAL1 PAL1 PR10A PR1B PR4 PSBA RBCL RBCS SGR SH11 SODA1 SODB SODCC1 SPL40 TIFY11A WRKY45 WRKY96 YGL98 _ _ _ | |
| INSD | - | |
| 系統 |
野生イネ コアコレクション |
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| 突然変異系統(遺伝研 Collection) | - | |
| 種子不稔系統 | - | |
| 致死変異体系統 | - | |
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組織別発生段階 - 突然変異系統(遺伝子) |
- | |
| 栽培イネ品種(遺伝研 Collection) | - | |
| 組織別発生段階 | - | |
