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Reference Detail
| Reference | ||
|---|---|---|
| Author | Liu E., Zeng S., Zhu S., Liu Y., Wu G., Zhao K., Liu X., Liu Q., Dong Z., Dang X., Xie H., Li D., Hu X., Hong D. | |
| Title | Favorable Alleles of <i>GRAIN-FILLING RATE1</i> Increase the Grain-Filling Rate and Yield of Rice. | |
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Abstract: Hybrid rice (<i>Oryza sativa</i>) has been cultivated commercially for 42 years in China. However, poor grain filling still limits the development of hybrid <i>japonica</i> rice. We report here the map-based cloning and characterization of the <i>GRAIN-FILLING RATE1</i> (<i>GFR1</i>) gene present at a major-effect quantitative trait locus. We elucidated and confirmed the function of <i>GFR1</i> via genetic complementation experiments and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) gene editing in combination with genetic and molecular biological analyses. In addition, we conducted haplotype association analysis to mine the elite alleles of <i>GFR1</i> among 117 rice accessions. We observed that <i>GFR1</i> was constitutively expressed and encoded a membrane-localized protein. The allele of the rice accession Ludao (<i>GFR1</i>Ludao) improved the grain-filling rate of rice by increasing Rubisco initial activity in the Calvin cycle. Moreover, the increased expression of the cell wall invertase gene <i>OsCIN1</i> in the near isogenic line NIL-<i>GFR1</i>Ludao promoted the unloading of Suc during the rice grain-filling stage. A yeast two-hybrid assay indicated that the Rubisco small subunit interacts with GFR1, possibly in the regulation of the rice grain-filling rate. Evaluation of the grain-filling rate and grain yield of F1 plants harboring <i>GFR1</i>Ludao and the alleles of 20 hybrids widely cultivated commercially confirmed that favorable alleles of <i>GFR1</i> can be used to further improve the grain-filling rate of hybrid <i>japonica</i> rice. |
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| Journal | Plant Physiol. | |
| Country | China | |
| Volume | 181(3) | |
| Pages | 1207-1222 | |
| Year | 2019 | |
| PubMed ID | 31519786 | |
| PubMed Central ID | 6836814 | |
| DOI | 10.1104/pp.19.00413 | |
| URL | - | |
| Relation | ||
| Gene | CIN1 CPD1 CPD2 GFR1 IAA10 IAA2 IAA20 RBCS RBCS1 RBCS3 RBCS4 RBCS5 SSI SSIIB SUT5 _ | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
|
Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
