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Reference Detail
| Reference | ||
|---|---|---|
| Author | Yi-Shih Chen, Tuan-Hua David Ho, Liu L., Ding Hua Lee, Chun-Hua Lee, Yi-Ru Chen, Shu-Yu Lin, Chung-An Lu, Su-May Yu | |
| Title | Sugar starvation-regulated MYBS2 and 14-3-3 protein interactions enhance plant growth, stress tolerance, and grain weight in rice. | |
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Abstract: Autotrophic plants have evolved distinctive mechanisms for maintaining a range of homeostatic states for sugars. The on/off switch of reversible gene expression by sugar starvation/provision represents one of the major mechanisms by which sugar levels are maintained, but the details remain unclear. alpha-Amylase (alphaAmy) is the key enzyme for hydrolyzing starch into sugars for plant growth, and it is induced by sugar starvation and repressed by sugar provision. <i>alphaAmy</i> can also be induced by various other stresses, but the physiological significance is unclear. Here, we reveal that the on/off switch of <i>alphaAmy</i> expression is regulated by 2 MYB transcription factors competing for the same promoter element. MYBS1 promotes <i>alphaAmy</i> expression under sugar starvation, whereas MYBS2 represses it. Sugar starvation promotes nuclear import of MYBS1 and nuclear export of MYBS2, whereas sugar provision has the opposite effects. Phosphorylation of MYBS2 at distinct serine residues plays important roles in regulating its sugar-dependent nucleocytoplasmic shuttling and maintenance in cytoplasm by 14-3-3 proteins. Moreover, dehydration, heat, and osmotic stress repress <i>MYBS2</i> expression, thereby inducing <i>alphaAmy3</i> Importantly, activation of <i>alphaAmy3</i> and suppression of <i>MYBS2</i> enhances plant growth, stress tolerance, and total grain weight per plant in rice. Our findings reveal insights into a unique regulatory mechanism for an on/off switch of reversible gene expression in maintaining sugar homeostatic states, which tightly regulates plant growth and development, and also highlight <i>MYBS2</i> and <i>alphaAmy3</i> as potential targets for crop improvement. |
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| Journal | Proc. Natl. Acad. Sci. U.S.A. | |
| Country | China | |
| Volume | 116(43) | |
| Pages | 21925-21935 | |
| Year | 2019 | |
| PubMed ID | 31594849 | |
| PubMed Central ID | 6815185 | |
| DOI | 10.1073/pnas.1904818116 | |
| URL | - | |
| Relation | ||
| Gene | AMY3D GF14B GF14C GF14D GF14G MYBS1 MYBS2 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
|
Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
