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| 文献 | ||
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| 著者 | Ni S., Li Z., Ying J., Zhang J., Chen H. | |
| タイトル | <i>Decreased Spikelets 4</i> Encoding a Novel Tetratricopeptide Repeat Domain-Containing Protein Is Involved in DNA Repair and spikelet number Determination in Rice. | |
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Abstract: spikelet number per panicle is a determinative factor of rice yield. DNA repair epigenetically alters the DNA accessibility, which can eventually regulate the transcription of the target genes. However, what and how DNA repair genes are related to rice spikelet development remains unknown. Here, we report the map-based cloning of a novel spikelet number gene <i>DES4</i> encoding a tetratricopeptide domain-containing protein. DES4 is a close ortholog of <i>Arabidopsis</i> BRU1, which is functionally related to axillary meristem development. A single base pair deletion in the last exon of <i>DES4</i> caused a premature stop of the resulting protein. The <i>DES4</i> mutant exhibited dwarf, reduced tiller, and spikelet numbers phenotypes, as well as hypersensitivity to genotoxic stresses, suggesting its essential role in DNA repair. <i>DES4</i> is predominantly expressed in young panicles and axillary meristems, and DES4 protein is localized in nucleus. A set of DNA repair genes such as <i>cyclins</i>, <i>KUs</i> (<i>KD subunits</i>) and <i>recombinases</i> were differentially regulated in <i>DES4</i>. Meanwhile, rice spikelet number genes <i>LAX1</i>, <i>LAX2</i>, and <i>MOC1</i> were significantly down-regulated in <i>DES4</i>. In morphology, <i>DES4</i> showed more severe reduction of spikelet numbers than <i>LAX1</i>, <i>lax2</i>, and <i>MOC1</i>, suggesting that <i>DES4</i> may work upstream of the three genes. |
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| 掲載誌 | Genes (Basel) | |
| Country | China | |
| 刊数 | 10(3) | |
| ページ | ||
| 発刊年 | 2019 | |
| PubMed ID | 30871267 | |
| PubMed Central ID | 6471630 | |
| DOI | 10.3390/genes10030214 | |
| URL | - | |
| 関連情報 | ||
| 遺伝子 | CYCA1;1 CYCA3;1 CYCD2;2 CYCU4;3 DES4 KU70 KU80 LAX1 LAX2 MOC1 RAD17 RAD50 RAD51A2 RAD51C | |
| INSD | - | |
| 系統 |
野生イネ コアコレクション |
- |
| 突然変異系統(遺伝研 Collection) | - | |
| 種子不稔系統 | - | |
| 致死変異体系統 | - | |
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組織別発生段階 - 突然変異系統(遺伝子) |
- | |
| 栽培イネ品種(遺伝研 Collection) | - | |
| 組織別発生段階 | - | |
