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Author Gong P., Luo Y., Huang F., Chen Y., Zhao C., Wu X., Li K., Yang X., Cheng F., Xiang X., Wu C., Pan G.
Title Disruption of a Upf1-like helicase-encoding gene OsPLS2 triggers light-dependent premature leaf senescence in rice.
Abstract:
The OsPLS2 locus was isolated and cloned by map-based cloning that encodes a Upf1-like helicase. Disruption of OsPLS2 accelerated light-dependent leaf senescence in the rice mutant of OsPLS2. leaf senescence is a very complex physiological process controlled by both genetic and environmental factors, however its underlying molecular mechanisms remain elusive. In this study, we report a novel Oryza sativa premature leaf senescence mutant (OsPLS2). Through map-based cloning, a G-to-A substitution was determined at the 1st nucleotide of the 13th intron in the OsPLS2 gene that encodes a Upf1-like helicase. This mutation prompts aberrant splicing of OsPLS2 messenger and consequent disruption of its full-length protein translation, suggesting a negative role of OsPLS2 in regulating leaf senescence. Wild-type rice accordingly displayed a progressive drop of OsPSL2 protein levels with age-dependent leaf senescence. Shading and light filtration studies showed that the OsPLS2 phenotype, which was characteristic of photo-oxidative stress and reactive oxygen species (ROS) accumulation, was an effect of irritation by light. When continuously exposed to far-red light, exogenous H2O2 and/or abscisic acid (ABA), the OsPLS2 mutant sustained hypersensitive leaf senescence. In consistence, light and ROS signal pathways in OsPLS2 were activated by down-regulation of PHYTOCHROME genes, and up-regulation of PHYTOCHROME-INTERACTING FACTORS (PIFs) and WRKY genes, all promoting leaf senescence. Together, these data indicated that OsPLS2 played an essential role in leaf senescence and its disruption triggered light-dependent leaf senescence in rice.
Journal Plant Mol. Biol.
Country China
Volume 100(1-2)
Pages 133-149
Year 2019
PubMed ID 30843130
PubMed Central ID -
DOI 10.1007/s11103-019-00848-4
URL -
Relation
Gene ABA8OX2 ABA8OX3 ELL ICL NCED1 NCED2 NCED3 PETA PHYA PLS2 PSAA PSAB PSAE PSBA PSBB PSBC PSBO RBCL RBCS RPOB SGR WRKY13 WRKY24 WRKY28 WRKY42 WRKY45 WRKY53 WRKY7 WRKY70 WRKY72 WRKY76 WRKY77 WRKY79 _ _ _
INSD -
Strain Wild Core Collection -
Induced Mutation Lines(NIG Collection) -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain
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Stages in Each Organ
- Muant Lines (Gene)
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Cultivated Varieties(NIG Collection) -
Stages in Each Organ -
/rice/oryzabase