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Reference Detail
| Reference | ||
|---|---|---|
| Author | Huang S., Liang Z., Chen S., Sun H., Fan X., Wang C., Xu G., Zhang Y. | |
| Title | A Transcription Factor, OsMADS57, Regulates Long-Distance Nitrate Transport and Root Elongation. | |
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Abstract: Root nitrate uptake adjusts to the plant's nitrogen demand for growth. Here, we report that OsMADS57, a MADS-box transcription factor, modulates nitrate translocation from rice (<i>Oryza sativa</i>) roots to shoots under low-nitrate conditions. <i>OsMADS57</i> is abundantly expressed in xylem parenchyma cells of root stele and is induced by nitrate. Compared with wild-type rice plants supplied with 0.2 mM nitrate, <i>OsMADS57</i> mutants had 31% less xylem loading of nitrate, while overexpression lines had 2-fold higher levels. Shoot-root 15N content ratios were 40% lower in the mutants and 76% higher in the overexpression lines. Rapid NO3- root influx experiments showed that mutation of <i>OsMADS57</i> did not affect root nitrate uptake. Reverse transcription quantitative PCR analysis of <i>OsNRT2</i> nitrate transporter genes showed that after 5 min in 0.2 mM nitrate, only <i>OsNRT2.3a</i> (a vascular-specific high-affinity nitrate transporter) had reduced (by two-thirds) expression levels. At 60 min of nitrate treatment, lower expression levels were also observed for three additional <i>NRT2</i> genes (<i>OsNRT2.1/2.2/2.4</i>). Conversely, in the overexpression lines, four <i>NRT2</i> genes had much higher expression profiles at all time points tested. As previously reported, <i>OsNRT2.3a</i> functions in nitrate translocation, indicating the possible interaction between <i>OsMADS57</i> and <i>OsNRT2.3a</i> Yeast one-hybrid and transient expression assays demonstrated that OsMADS57 binds to the CArG motif (CATTTTATAG) within the <i>OsNRT2.3a</i> promoter. Moreover, seminal root elongation was inhibited in <i>OsMADS57</i> mutants, which may be associated with higher auxin levels in and auxin polar transport to root tips of mutant plants. Taken together, these results suggest that OsMADS57 has a role in regulating nitrate translocation from root to shoot via OsNRT2.3a. |
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| Journal | Plant Physiol. | |
| Country | China | |
| Volume | 180(2) | |
| Pages | 882-895 | |
| Year | 2019 | |
| PubMed ID | 30886113 | |
| PubMed Central ID | 6548263 | |
| DOI | 10.1104/pp.19.00142 | |
| URL | - | |
| Relation | ||
| Gene | HTNE MADS57 NRT2.1 NRT2.2 NRT2.4 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
