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Reference Detail
| Reference | ||
|---|---|---|
| Author | Chen C., He B., Liu X., Ma X., Liu Y., Yao H.Y., Zhang P., Yin J., Wei X., Koh H.J., Yang C., Xue H.W., Fang Z., Qiao Y. | |
| Title | Pyrophosphate-fructose 6-phosphate 1-phosphotransferase (PFP1) regulates starch biosynthesis and seed development via heterotetramer formation in rice (Oryza sativa L.). | |
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Abstract: Pyrophosphate-fructose 6-phosphate 1-phosphotransferase (PFP1) reversibly converts fructose 6-phosphate and pyrophosphate to fructose 1, 6-bisphosphate and orthophosphate during glycolysis, and has diverse functions in plants. However, mechanisms underlying the regulation of starch metabolism by PFP1 remain elusive. This study addressed the function of PFP1 in rice floury endosperm and defective grain filling. Compared with the wild type, PFP1-3 exhibited remarkably low grain weight and starch content, significantly increased protein and lipid content, and altered starch physicochemical properties and changes in embryo development. Map-based cloning revealed that PFP1-3 is a novel allele and encodes the regulatory beta-subunit of PFP1 (PFP1beta). Measurement of nicotinamide adenine dinucleotide (NAD+) showed that mutation of PFP1beta markedly decreased its enzyme activity. PFP1beta and three of four putative catalytic alpha-subunits of PFP1, PFP1alpha1, PFP1alpha2, and PFP1alpha4, interacted with each other to form a heterotetramer. Additionally, PFP1beta, PFP1alpha1 and PFP1alpha2 also formed homodimers. Furthermore, transcriptome analysis revealed that mutation of PFP1beta significantly altered expression of many essential enzymes in starch biosynthesis pathways. Concentrations of multiple lipid and glycolytic intermediates and trehalose metabolites were elevated in PFP1-3 endosperm, indicating that PFP1 modulates endosperm metabolism, potentially through reversible adjustments to metabolic fluxes. Taken together, these findings provide new insights into seed endosperm development and starch biosynthesis and will help in the breeding of rice cultivars with higher grain yield and quality. |
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| Journal | Plant Biotechnol. J. | |
| Country | China | |
| Volume | ||
| Pages | ||
| Year | 2019 | |
| PubMed ID | 31131526 | |
| PubMed Central ID | - | |
| DOI | 10.1111/pbi.13173 | |
| URL | - | |
| Relation | ||
| Gene | AGPL1 AGPL2 ALK FLO5 ISA2 ISA3 PFPA1 PFPA2 PFPA4 PFPB SBE3 SBE4 SSI SSIIB SSIVB WX1 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
