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Reference Detail
| Reference | ||
|---|---|---|
| Author | Guo T., Wang D., Fang J., Zhao J., Yuan S., Xiao L., Li X. | |
| Title | Mutations in the Rice <i>OsCHR4</i> Gene, Encoding a CHD3 Family Chromatin Remodeler, Induce Narrow and Rolled Leaves with Increased Cuticular Wax. | |
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Abstract: Leaf blade width, curvature, and cuticular wax are important agronomic traits of rice. Here, we report the rice <i>OsCHR4-5</i> mutant characterized by pleiotropic phenotypes, including narrow and rolled leaves, enhanced cuticular wax deposition and reduced plant height and tiller number. The reduced leaf width is caused by a reduced number of longitudinal veins and increased auxin content. The cuticular wax content was significantly higher in the <i>OsCHR4-5</i> mutant, resulting in reduced water loss rate and enhanced drought tolerance. Molecular characterization reveals that a single-base deletion results in a frame-shift mutation from the second chromodomain of OsCHR4, a CHD3 (chromodomain helicase DNA-binding) family chromatin remodeler, in the <i>OsCHR4-5</i> mutant. Expressions of seven wax biosynthesis genes (<i>GL1-4</i>, <i>WSL4</i>, <i>OsCER7</i>, <i>LACS2</i>, <i>LACS7</i>, <i>ROC4</i> and <i>BDG</i>) and four auxin biosynthesis genes (<i>YUC2</i>, <i>YUC3</i>, <i>YUC5</i> and <i>YUC6</i>) was up-regulated in the <i>OsCHR4-5</i> mutant. Chromatin immunoprecipitation assays revealed that the transcriptionally active histone modification H3K4me3 was increased, whereas the repressive H3K27me3 was reduced in the upregulated genes in the <i>OsCHR4-5</i> mutant. Therefore, <i>OsCHR4</i> regulates leaf morphogenesis and cuticle wax formation by epigenetic modulation of auxin and wax biosynthetic genes expression. |
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| Journal | Int J Mol Sci | |
| Country | China | |
| Volume | 20(10) | |
| Pages | ||
| Year | 2019 | |
| PubMed ID | 31130602 | |
| PubMed Central ID | 6566577 | |
| DOI | 10.3390/ijms20102567 | |
| URL | - | |
| Relation | ||
| Gene | BDG CER7 CHR705 DLN32 DLN39 HMS1 LACS2 LACS7 LTR1 PKL RFS ROC4 YUCCA2 YUCCA3 YUCCA5 YUCCA6 _ | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
