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Author Hao Y., Wang Y., Wu M., Zhu X., Teng X., Sun Y., Zhu J., Zhang Y., Jing R., Lei J., Li J., Bao X., Wang C., Wang Y., Wan J.
Title A nucleus-localized PPR protein OsNPPR1 is important for mitochondrial function and endosperm development in rice.
Abstract:
Pentatricopeptide repeat (PPR) proteins constitute one of the largest protein families in land plants. Recent studies revealed the functions of PPR proteins in organellar RNA metabolism and plant development, but the functions of most PPR proteins, especially PPRs localized in the nucleus, remain largely unknown. Here, we report the isolation and characterization of a rice mutant named floury and growth retardation1 (fgr1). fgr1 showed floury endosperm with loosely arranged starch grains, decreased starch and amylose contents, and retarded seedling growth. Map-based cloning showed the mutant phenotype was caused by a single nucleotide substitution in the coding region of Os08g0290000. This gene encodes a nucleus-localized PPR protein we named it OsNPPR1 that affected mitochondrial function. In vitro SELEX and RNA-EMSA assays showed that OsNPPR1 was an RNA protein that bound to the CUCAC motif determined. Moreover, a number of retained intron (RI) events were detected in fgr1. Thus, OsNPPR1 was involved in regulation of mitochondrial development and/or functions that are important for endosperm development. Our results provide novel insights into coordinated interaction between nucleus-localized PPR proteins and mitochondrial function.
Journal J. Exp. Bot.
Country China
Volume
Pages
Year 2019
PubMed ID 31087099
PubMed Central ID -
DOI 10.1093/jxb/erz226
URL -
Relation
Gene FGR1 FLO2 MICU1 MORC6C TWI1 _ _
INSD -
Strain Wild Core Collection -
Induced Mutation Lines(NIG Collection) -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain
-
Stages in Each Organ
- Muant Lines (Gene)
-
Cultivated Varieties(NIG Collection) -
Stages in Each Organ -
/rice/oryzabase