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Reference Detail
Reference | ||
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Author | Monna L., Kitazawa N., Yoshino R., Suzuki J., Masuda H., Maehara Y., Tanji M., Sato M., Nasu S., Minobe Y. | |
Title | Positional cloning of rice semidwarfing gene, sd-1: rice "green revolution gene" encodes a mutant enzyme involved in gibberellin synthesis. | |
Abstract: A rice semidwarfing gene, sd-1, known as the "green revolution gene," was isolated by positional cloning and revealed to encode gibberellin 20-oxidase, the key enzyme in the gibberellin biosynthesis pathway. Analysis of 3477 segregants using several PCR-based marker technologies, including cleaved amplified polymorphic sequence, derived-CAPS, and single nucleotide polymorphisms revealed 1 ORF in a 6-kb candidate interval. Normal-type rice cultivars have an identical sequence in this region, consisting of 3 exons (558, 318, and 291 bp) and 2 introns (105 and 1471 bp). Dee-Geo-Woo-Gen-type sd-1 mutants have a 383-bp deletion from the genome (278-bp deletion from the expressed sequence), from the middle of exon 1 to upstream of exon 2, including a 105-bp intron, resulting in a frame-shift that produces a termination codon after the deletion site. The radiation-induced sd-1 mutant Calrose 76 has a 1-bp substitution in exon 2, causing an amino acid substitution (Leu [CTC] to Phe [TTC]). Expression analysis suggests the existence of at least one more locus of gibberellin 20-oxidase which may prevent severe dwarfism from developing in sd-1 mutants. |
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Journal | DNA Res. | |
Country | Japan | |
Volume | 9(1) | |
Pages | 11-7 | |
Year | 2002 | |
PubMed ID | 11939564 | |
PubMed Central ID | - | |
DOI | 10.1093/dnares/9.1.11 | |
URL | - | |
Relation | ||
Gene | SD1 | |
INSD | - | |
Strain | Wild Core Collection | - |
Induced Mutation Lines(NIG Collection) | - | |
Sterile Seed Strain | - | |
Lethal Embryo Mutantion Strain |
- | |
Stages in Each Organ - Muant Lines (Gene) |
- | |
Cultivated Varieties(NIG Collection) | - | |
Stages in Each Organ | - |