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Reference Detail
| Reference | ||
|---|---|---|
| Author | Thangasamy S., Cian-Ling Guo, Ming-Hsiang Chuang, Ming-Hsing Lai, Chen J., Guang-Yuh Jauh | |
| Title | Rice SIZ1, a SUMO E3 ligase, controls spikelet fertility through regulation of anther dehiscence. | |
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Abstract: • Sumoylation, a post-translational modification, has important functions in both animals and plants. However, the biological function of the SUMO E3 ligase, SIZ1, in rice (Oryza sativa) is still under investigation. • In this study, we employed two different genetic approaches, the use of SIZ1 T-DNA mutant and SIZ1-RNAi transgenic plants, to characterize the function of rice SIZ1. • Genetic results revealed the co-segregation of single T-DNA insertional recessive mutation with the observed phenotypes in SIZ1. In addition to showing reduced plant height, tiller number and seed set percentage, both the SIZ1 mutant and SIZ1-RNAi transgenic plants showed obvious defects in anther dehiscence, but not pollen viability. The anther indehiscence in SIZ1 was probably a result of defects in endothecium development before anthesis. Interestingly, rice orthologs of AtIRX and ZmMADS2, which are essential for endothecium development during anther dehiscence, were significantly down-regulated in SIZ1. Compared with the wild-type, the sumoylation profile of high-molecular-weight proteins in mature spikelets was reduced significantly in SIZ1 and the SIZ1-RNAi line with notably reduced SIZ1 expression. The nuclear localization signal located in the SIZ1 C-terminus was sufficient for its nuclear targeting in bombarded onion epidermis. • The results suggest the functional role of SIZ1, a SUMO E3 ligase, in regulating rice anther dehiscence. |
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| Journal | New Phytol. | |
| Country | Taiwan | |
| Volume | 189(3) | |
| Pages | 869-82 | |
| Year | 2011 | |
| PubMed ID | 21083564 | |
| PubMed Central ID | - | |
| DOI | 10.1111/j.1469-8137.2010.03538.x | |
| URL | - | |
| Relation | ||
| Gene | CCR21 MADS27 SIZ1 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
