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| 著者 | Ma J., Wang Y., Ma X., Meng L., Jing R., Wang F., Wang S., Cheng Z., Zhang X., Jiang L., Wang J., Wang J., Zhao Z., Guo X., Lin Q., Wu F., Zhu S., Wu C., Ren Y., Lei C., Zhai H., Wan J. | |
| タイトル | Disruption of gene SPL35, encoding a novel CUE domain-containing protein, leads to cell death and enhanced disease response in rice. | |
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Abstract: lesion mimic mutants that exhibit spontaneous hypersensitive response (HR)-like necrotic lesions are ideal experimental systems for elucidating molecular mechanisms involved in plant cell death and defence responses. Here we report identification of a rice lesion mimic mutant, spotted leaf 35 (SPL35), and cloning of the causal gene by TAIL-PCR strategy. SPL35 exhibited decreased chlorophyll content, higher accumulation of H2 O2 , up-regulated expression of defence-related marker genes, and enhanced resistance to both fungal and bacterial pathogens of rice. The SPL35 gene encodes a novel CUE (coupling of ubiquitin conjugation to ER degradation) domain-containing protein that is predominantly localized in cytosol, ER and unknown punctate compartment(s). SPL35 is constitutively expressed in all organs, and both overexpression and knockdown of SPL35 cause the lesion mimic phenotype. SPL35 directly interacts with the E2 protein OsUBC5a and the coatomer subunit delta proteins Delta-COP1 and Delta-COP2 through the CUE domain, and down-regulation of these interacting proteins also cause development of HR-like lesions resembling those in SPL35 and activation of defence responses, indicating that SPL35 may be involved in the ubiquitination and vesicular trafficking pathways. Our findings provide insight into a role of SPL35 in regulating cell death and defence response in plants. |
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| 掲載誌 | Plant Biotechnol. J. | |
| Country | China | |
| 刊数 | ||
| ページ | ||
| 発刊年 | 2019 | |
| PubMed ID | 30771255 | |
| PubMed Central ID | - | |
| DOI | 10.1111/pbi.13093 | |
| URL | - | |
| 関連情報 | ||
| 遺伝子 | AOS2 BIP1 CHS CNX DELTA-COP1 DELTA-COP2 PAL1 PDIL1-1 PR10A PR1B PR5 SPL35 UBC5A | |
| INSD | - | |
| 系統 |
野生イネ コアコレクション |
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| 突然変異系統(遺伝研 Collection) | - | |
| 種子不稔系統 | - | |
| 致死変異体系統 | - | |
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組織別発生段階 - 突然変異系統(遺伝子) |
- | |
| 栽培イネ品種(遺伝研 Collection) | - | |
| 組織別発生段階 | - | |
